165,343 results

1. hNTa2-qgRNA-pYJA5

   Plasmid

   #217780

   Purpose

   Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Insert

   Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   human U6, mouse U6, human H1, human 7SK

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pECFP18aaEYFP

   Plasmid

   #109330

   Purpose

   ECFP-EYFP FRET positive control

   Depositor

   Gabriele Kaminski Schierle

   Article

   Kaminski et al Methods Mol Biol. 2014;1076:445-54. doi:

   Insert

   ECFP-EYFP FRET positive control
   

   Expression

   Mammalian

   Mutation

   ECFP linked to EYFP with 18 aa linker GLRSRAQASNS…

   Promoter

   CMV

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. N-Terminal Split Cas9 with GyrA intein

   Plasmid

   #58693

   Purpose

   Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
   

   Use

   AAV and CRISPR

   Tags

   3xFlag, GyrA Nsplit Intein, and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pgk-ATG-frt

   Plasmid

   #20734

   Depositor

   Rudolf Jaenisch

   Article

   Beard et al Genesis. 2006 Jan . 44(1):23-8.

   Insert

   pgk-ATG-frt-SAdpA-tetO
   

   Use

   Mouse Targeting

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAX APP C99

   Plasmid

   #30146

   Depositor

   Dennis Selkoe, Tracy Young-Pearse

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   APP C99 (APP Human)
   

   Expression

   Mammalian

   Mutation

   C-terminal from BACE site of APP

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFPC2-Gephyrin P1

   Plasmid

   #68815

   Purpose

   expression of fluorescent tagged Geph in mammalian cells

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   silent mutations on internal EcoRI site. Gephyri…

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. GST-HA-GFP11-N1

   Plasmid

   #91953

   Purpose

   Expresses GST-HA-GFP11 in mammalian cells

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   GST-HA-GFP11
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-p18-FLAG

   Plasmid

   #42331

   Depositor

   David Sabatini

   Article

   Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

   Insert

   p18 (LAMTOR1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPSup_iGFP

   Plasmid

   #149420

   Purpose

   Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter. The GFP reporter gene harbors the IV2 intron.

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

    Plasmid

    #126552

    Purpose

    Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

    Depositor

    Jonathan Karn

    Article

    Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

    Insert

    HIV-1 vector pNL4-3
    

    Use

    Lentiviral

    Tags

    CD8a and GFP

    Mutation

    Delta gag/pol

    Promoter

    HIV LTR

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. 5xUAS:GAP-EGFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid

    #158652

    Purpose

    making transgenics expressing a UAS driven eGFP and NTR 2.0

    Depositor

    Jeff Mumm

    Article

    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

    Insert

    5xUAS:GAP-eGFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
    

    Use

    Tol2-based transgenic expression in zebrafish/oth…

    Mutation

    F70A/F108Y

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti_gRNA-Puro

    Plasmid

    #84752

    Purpose

    Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

    Plasmid

    #234951

    Purpose

    Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMTS-HA-GFP11

    Plasmid

    #177725

    Purpose

    Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    MTS-HA-GFP11
    

    Tags

    GFP11 and HA

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3662 HPV-16 E2

    Plasmid

    #22457

    Depositor

    Peter Howley

    Article

    Sakai et al J Virol. 1996 Mar . 70(3):1602-11.

    Insert

    E2 WT
    

    Expression

    Mammalian

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pP2_tail_fiber

    Plasmid

    #196332

    Purpose

    Plasmid expressing P2 tail fiber H and chaperone protein G

    Depositor

    Baojun Wang

    Article

    Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

    Insert

    P2 tail fiber H and chaperone protein G
    

    Expression

    Bacterial

    Promoter

    P2 late promoter

    Available Since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMBP-MS2

    Plasmid

    #65104

    Purpose

    can be used to isolate RNA binding complexes

    Depositor

    Josep Vilardell

    Article

    Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

    Insert

    MS2
    

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    double mutation (V75Q and A81G) that prevents oli…

    Promoter

    tac

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. HAP40 1-371

    Plasmid

    #124060

    Purpose

    Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HAP40 (F8A1 Human)
    

    Use

    Baculovirus expression

    Tags

    6x His and TEV cleavage site

    Promoter

    polyhedrin

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-fDIO-mNeonGreen

    Plasmid

    #99133

    Purpose

    An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    fDIO-mNeonGreen
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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