161,273 results

1. pGEx-4T-1-YTHDF3

   Plasmid

   #70088

   Purpose

   Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pTipi2.1_Anti-GCN4 [IPI-C11L34] Mouse/Rabbit HC

   Plasmid

   #221353

   Purpose

   Mammalian expression of Anti-GCN4 [IPI-C11L34] Mouse/Rabbit HC; to be used with Anti-GCN4 [IPI-C11L34] light chain (Plasmid 221354) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-GCN4 [IPI-C11L34] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. Mito-TurboID

   Plasmid

   #200960

   Purpose

   Transiently expresses mitochondria localized TurboID with V5 tags

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   mito-TurboID
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-GRAM-H

   Plasmid

   #211706

   Purpose

   Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H) in mammalian cells

   Depositor

   Yasunori Saheki

   Article

   Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

   Insert

   B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
   

   Use

   Tags

   ddFP-B3

   Expression

   Mammalian

   Mutation

   Changed Glycine 187 to Leucine

   Promoter

   CMV

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

   Plasmid

   #197502

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

   Promoter

   CMV and T7

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCmini-iCAP-PHP.N

   Plasmid

   #127851

   Purpose

   non-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

   Insert

   Synthetic construct isolate AAV-PHP.N VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR26 CAG AsiSI/MluI

   Plasmid

   #74286

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. bNS2B47NS3

   Plasmid

   #155320

   Purpose

   co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-hLC3B-pcDNA3.1

   Plasmid

   #40827

   Depositor

   David Rubinsztein

   Article

   Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. LRG2.1

    Plasmid

    #108098

    Purpose

    Lentiviral expression plasmid of sgRNA with GFP

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE31-rsEGFP2

    Plasmid

    #102879

    Purpose

    Expresses rsEGFP2 in bacterial cells

    Depositor

    Stefan Jakobs

    Article

    Grotjohann et al elife. 2012;1:e00248. doi: 10.7554/eLife

    Insert

    rsEGFP2
    

    Use

    Tags

    6xHisTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWPXL-Sox9

    Plasmid

    #36979

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    Sox9 (Sox9 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. psiCheck2-SV40p-3xFLAG-HSVTKp-mCherry

    Plasmid

    #182377

    Purpose

    Dual expression construct encoding 3xFLAG and mCherry from separate promoters

    Depositor

    Chunghun Lim

    Article

    Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

    Inserts

    3xFlag
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HSV TK promoter and SV40 promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-Stat3-C

    Plasmid

    #13373

    Purpose

    Retroviral expression of dominant active mouse Stat3

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    signal transducer and activator of transcription 3 (dominant active mutant) (Stat3 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed Alanine 661 to cysteine and asparagine 66…

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. GW1-PercevalHR

    Plasmid

    #49082

    Purpose

    Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CBh-DIO-EGFP

    Plasmid

    #87168

    Purpose

    Expression of eGFP in the presence of Cre

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    EGFP
    

    Use

    AAV

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. 013LP-pAAV-ANF-657-EmGFP-pA

    Plasmid

    #120267

    Purpose

    AAV vector for Atrial specific EmGFP expression

    Depositor

    Xander Wehrens

    Article

    Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

    Insert

    EmGFP
    

    Use

    AAV

    Tags

    3XFlag and HA

    Expression

    Mutation

    Promoter

    ANF promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid

    #195316

    Purpose

    Dox inducible lentiviral dead Cas9

    Depositor

    Raj Chari

    Insert

    dead Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    D10A; H840A

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

    Plasmid

    #162379

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPR-aBEST

    Plasmid

    #131464

    Purpose

    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

    Inserts

    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    tipA

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge