pcDNA3.1-(-)-Pur
(Plasmid
#200458)
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Purpose(Empty Backbone) Eukaryotic expression vector
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 200458 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerThermofisher
- Backbone size (bp) 5185
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Modifications to backboneThe initial vector was pcDNA3.1-(-)-Hyg. The Hygromycin ORF was replace with the Puromycin ORF amplified from Promega pGL4.22-Pur. The final vector was obtained by PCR fragments assembly.
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Original backbone from Thermofisher (pcDNA3.1-(-)-Hyg, insert amplified from Promega pGL4.22-Pur
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-(-)-Pur was a gift from Bertrand Collet (Addgene plasmid # 200458 ; http://n2t.net/addgene:200458 ; RRID:Addgene_200458)
