p5068 pGEX-6P-1 Brd4 full-length
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14447||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameBrd 4
SpeciesH. sapiens (human)
Insert Size (bp)4100
Entrez GeneBRD4 (a.k.a. CAP, HUNK1, HUNKI, MCAP)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pGEX 5'
- 3′ sequencing primer pGEX 3' (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p5068 pGEX-6P-1 Brd4 full-length was a gift from Peter Howley (Addgene plasmid # 14447 ; http://n2t.net/addgene:14447 ; RRID:Addgene_14447)
For your References section:Interaction of the bovine papillomavirus E2 protein with Brd4 tethers the viral DNA to host mitotic chromosomes. You J, Croyle JL, Nishimura A, Ozato K, Howley PM. Cell. 2004 Apr 30. 117(3):349-60. 10.1016/S0092-8674(04)00402-7 PubMed 15109495
Map uploaded by the depositor.