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Depositing Lab
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Depositing OrganizationUniversity of California, Los Angeles (UCLA)
Located in United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20013 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 20013-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 20013-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonecFlag pcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5-alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namec-Rel
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Alt nameRel
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SpeciesM. musculus (mouse)
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Insert Size (bp)1767
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GenBank IDNM_009044
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Entrez GeneRel (a.k.a. c-Rel)
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Tag
/ Fusion Protein
- Flag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See cFlag pcDNA plasmid for backbone information. 5' insert restriction site flanked by kozak/start site (CCACCATG).
Note from depositing lab: c-Rel is expressed at a lower level than other NF-kB family members.
DNA (Catalog # 20013-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 20013-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
c-Rel cFlag pcDNA3 was a gift from Stephen Smale (Addgene plasmid # 20013 ; http://n2t.net/addgene:20013 ; RRID:Addgene_20013) -
For your References section:
A c-Rel subdomain responsible for enhanced DNA-binding affinity and selective gene activation. Sanjabi S, Williams KJ, Saccani S, Zhou L, Hoffmann A, Ghosh G, Gerondakis S, Natoli G, Smale ST. Genes Dev. 2005 Sep 15. 19(18):2138-51. 10.1101/gad.1329805 PubMed 16166378