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FUmGW
(Plasmid #22479)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22479 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    FUGW
  • Backbone manufacturer
    I. Verma, Salk
  • Backbone size w/o insert (bp) 9165
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    membrane-bound green fluorescent protein
  • Alt name
    mGFP
  • Alt name
    GFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    799
  • Tag / Fusion Protein
    • palmitoylation (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI, BamHI, AgeI, (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUmGW was a gift from Connie Cepko (Addgene plasmid # 22479 ; http://n2t.net/addgene:22479 ; RRID:Addgene_22479)
  • For your References section:

    Retinal progenitor cells can produce restricted subsets of horizontal cells. Rompani SB, Cepko CL. Proc Natl Acad Sci U S A. 2008 Jan 8. 105(1):192-7. 10.1073/pnas.0709979104 PubMed 18162542