pABCb10-GFP
(Plasmid #19031)

• Depositing Lab: Orian Shirihai
• Publication: Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-63.

Backbone

• Vector backbone: pIRES
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6092
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ATP-binding cassette, sub-family B (MDR/TAP), member 10
• Alt name: ABCme
• Alt name: ABCb10
• Alt name: Abc-mitochondrial erythroid
• Species: M. musculus (mouse)
• Insert Size (bp): 2147
• GenBank ID: NM_019552
• Entrez Gene: Abcb10 (a.k.a. AU016331, Abc-me, Abcb12, C76534)
• Tag / Fusion Protein:
  • HIS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: MluI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: IRES-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry