167,150 results
-
Plasmid#138966PurposePeft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegansDepositorInsertwrmScarlet1-10
ExpressionWormAvailable SinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pTEF_Cas9
Plasmid#104909PurposehCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selectionDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable SinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN019-SpCas9-hiNLS-t-M1M4
Plasmid#234456PurposeExpresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGGDepositorInsertSpCas9 hiNLS t-M1M4
UseCRISPRTagsHis6, Nucleoplasmin, SV40, and c-MycExpressionBacterialMutationCys at positions 80 and 574 have been mutated to …Available SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF2307
Plasmid#142023PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pESR1.1.0-gDNA
Plasmid#112433PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1DepositorAvailable SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC22A6_STOP
Plasmid#161131PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC22A6 (SLC22A6 Human)
ExpressionMammalianAvailable SinceJune 21, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV.CAG.LSL.tdTomato
Plasmid#100048PurposeAAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)DepositorInserttdtomato
UseAAV and Cre/LoxExpressionMammalianPromoterCAGAvailable SinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbOcIgG-Tn5
Plasmid#184285PurposeAnti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)DepositorInsertnbOcIgG-Tn5
Available SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMetYC-Dest
Plasmid#105081PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.DepositorTypeEmpty backboneTagsCub-PLVExpressionYeastAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-NHK-MYC-His6
Plasmid#185350PurposeAssessing of ubiquitination in cellsDepositorInserta1-antitrypsin Hong Kong Null mutant
TagsMyc, 6xHisExpressionMammalianMutationNHK mutantAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-Ace-mNeon2-Kv2.1PR
Plasmid#195528PurposeGreen fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targetedDepositorInsertAce-mNeon2-Kv2.1 proximal restriction sequence
UseAAVExpressionMammalianMutationAce-mNeon 81S; SY linkerPromoterCaMKIIAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNSEN-d2
Plasmid#59763PurposeExpresses nuclear EGFP and Tomato in mammalian cellsDepositorInsertsd2-EGFP
Tomato
Tags3X NLS and PESTExpressionMammalianPromoterCMV promoter and SV40 promoterAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-EGFP
Plasmid#50469PurposeEGFP under the control of CaMKIIa promoterDepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEGFP
UseAAVTagsN/APromoterCaMKIIaAvailable SinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPBCAG-rtTAM2-IH
Plasmid#140827PurposeExpresses rtTAM2-IRES-HygR in mammalian cellsDepositorInsertsrtTAM2
HygR
ExpressionMammalianAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNF02-mLemon
Plasmid#219399PurposeConstitutive expression of mLemon in E. coliDepositorInsertmLemon
ExpressionBacterialPromoterproDpAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Dlx-FLEX-hM4Di-mCherry
Plasmid#209701PurposeExpresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent mannerDepositorInserthM4D(Gi)
UseAAVTagsmCherryAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tn7 integration plasmid
Plasmid#154134PurposeSuicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.DepositorInsertsleft and right end of Tn7 arm
Spectinomycin resistant gene
ExpressionBacterialPromoterSpectinomycin resistance gene promoter and noAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEHAC1-HA-BirAonly
Plasmid#232584PurposeExpress HA-BirADepositorInsertBirA
TagsHAExpressionMammalianAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
LV-AR-KKKAAA
Plasmid#171219PurposeLenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.DepositorInsertAR-KKKAAA (AR Human)
UseLentiviralTagsFlagExpressionMammalianMutationAR-K630A-K632A-K633APromoterEF-1aAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE-GFP-Blast
Plasmid#135673PurposeDox-inducible expression (TRE promoter) of GFP cDNA (control for other cDNAs), Blasticidin selectionDepositorInsertGFP
UseLentiviralAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only