161,398 results

1. pTn Donor

   Plasmid

   #236211

   Purpose

   Arabinose-inducible Tn5 InducTn-seq plasmid

   Depositor

   Matthew Waldor

   Article

   Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

   Insert

   None
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG/ATP11C-HA

   Plasmid

   #209220

   Purpose

   Mammalian expression of ATP11C

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP11C (ATP11C Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-scFv-T

   Plasmid

   #67843

   Purpose

   pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem of in-frame amber codons in scFvs.

   Depositor

   Joanna Bereta

   Article

   Ossysek et al Immunol Lett. 2015 Jul 25. pii: S0165-24

   Insert

   antibody 1D8 light kappa chain
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-GSDMD-eGFP-SV40p-BlasR

   Plasmid

   #228254

   Purpose

   pLenti-CMV-GSDMD-eGFP-SV40p-BlasR plasmid for ectopic expression of human full-length GSDMD fusion with GFP

   Depositor

   Judy Lieberman

   Article

   Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

   Insert

   GSDMD (GSDMD Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Expotin 1

   Plasmid

   #42047

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   Exportin-1 (XPO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213029

   Purpose

   Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYMA3_SidK_GFP

   Plasmid

   #184276

   Purpose

   Legionella pneumophila SidK (1-278) - GFP

   Depositor

   John Rubinstein

   Article

   Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

   Insert

   SidK (1-278) (sidK Legionella pneumophila)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti6/V5-p53_R175H

   Plasmid

   #22936

   Depositor

   Bernard Futscher

   Article

   Junk et al Neoplasia. 2008 May . 10(5):450-61.

   Insert

   tumor protein 53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   R175H Mutant

   Promoter

   Available since

   March 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. TMEM106B-EGFP

   Plasmid

   #210366

   Purpose

   Expresses TMEM106B fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   TMEM106B (TMEM106B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-FOXP3

    Plasmid

    #153147

    Purpose

    Expression of Flag tagged human FOXP3 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXP3 (FOXP3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS159

    Plasmid

    #122378

    Purpose

    CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-6xGLY-3xFLAG-HIS3MX6

    Plasmid

    #20753

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNatB (pACYCduet-naa20-naa25)

    Plasmid

    #53613

    Purpose

    Allows expression of the fission yeast NatB complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

    Inserts

    naa20 (naa20 Fission Yeast)
    naa25 (naa25 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDV002

    Plasmid

    #191823

    Purpose

    Plasmid for the transformation of the cyanobacterium Arthrospira platensis at the NS1 locus. No promoter. No terminator. Golden Gate compatible. Selection: in A. platensis: aadA; E. coli: AmpR

    Depositor

    Jim Roberts

    Article

    Tabakh et al STAR Protoc. 2023 Feb 8;4(1):102087. doi

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-sfGFP

    Plasmid

    #85492

    Purpose

    pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-N-Blast-ultraID

    Plasmid

    #207682

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. HIP-flash (HOP-flash mutant)

    Plasmid

    #83466

    Purpose

    Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

    Depositor

    Barry Gumbiner

    Article

    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

    Insert

    Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-tmpknot-GG-acceptor

    Plasmid

    #174039

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_AncBE4max

    Plasmid

    #112094

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    AncBE4max
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.3_d2eGFP

    Plasmid

    #26821

    Depositor

    Derrick Rossi

    Article

    Warren et al Cell Stem Cell. 2010 Sep 29. ():.

    Insert

    5'UTR-d2eGFP-3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    destabilized (PEST sequence) GFP

    Promoter

    Available since

    Nov. 17, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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