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CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming.
Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS
Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 10.1016/j.stem.2018.09.003.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
118587TetO-FUW-Ezh2Expresses Ezh2 from the TRE3G promoter
118588TetO-FUW-Foxo1Expresses Foxo1 from the TRE3G promoter
118589TetO-FUW-Ngn1Expresses Ngn1 from the TRE3G promoter
118590TetO-FUW-Mecom 1Expresses Mecom isoform 1 from the TRE3G promoter
118591TetO-FUW-Mecom 2Expresses Mecom isoform 2 from the TRE3G promoter
118592pHR-EF1Alpha-Tet-on 3GExpresses Tet-on 3G from the EF1Alpha promoter
118593pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter
118594pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFPParental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter
122132pHR-TRE3G-dCas9-10X GCN4-P2A-mCherryExpress a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoter
122133pHR-PGK-scFv GCN4-sfGFP-VP64Express a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter

Antibodies from Article