|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102962||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5675
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsNEB Shuffle T7 recommended for protein expression
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site Kpn 1 (not destroyed)
- 3′ cloning site Nhe 1 (not destroyed)
- 5′ sequencing primer GGTCGTCAGACTGTCGATGAAGCC
- 3′ sequencing primer TGTCCTACTCAGGAGAGCGTTCAC (Common Sequencing Primers)
In the presence of IPTG and biotin, bacteria will generate MBP/biotinylated fusion protein.
MBP fusion protein with C-terminal MCS-TEV-AviTAG-HIS
Vector expresses BirA (Biotin ligase) from E.coli.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMal-T-Avi-His/BirA was a gift from Tonia Rex (Addgene plasmid # 102962 ; http://n2t.net/addgene:102962 ; RRID:Addgene_102962)