VPS13A^mCherry
(Plasmid #118758)

• Purpose: VPS13A internally tagged with mCherry at residue 1372.
• Depositing Lab: Pietro De Camilli
• Publication: Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-3639. doi: 10.1083/jcb.201807019. Epub 2018 Aug 9.

Backbone

• Vector backbone: pEGFP-N1
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 14000
• Modifications to backbone: Excised EGFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Grow at 32 degrees Celsius increasing time of growth by 50%
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: VPS13A
• Alt name: Chorein
• Species: H. sapiens (human)
• Insert Size (bp): 10000
• Entrez Gene: VPS13A (a.k.a. BLTP5A, CHAC, CHOREIN)
• Tag / Fusion Protein:
  • mCherry inserted at position 1372

Resource Information

• Supplemental Documents:
  • ApE file of plasmid sequence
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry