166,201 results

1. TurboID-His6_pET21a

   Plasmid

   #107177

   Purpose

   expresses His6-tagged TurboID in bacteria

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   TurboID (BirA mutant)
   

   Expression

   Bacterial

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-BRD4

   Plasmid

   #65378

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD4 (BRD4 Human)
   

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   R1232W

   Promoter

   CMV

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. GfaABC1D BARK D110A p2A mCherry

   Plasmid

   #117692

   Purpose

   GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Use

   AAV

   Tags

   HA / FLAG

   Mutation

   D110A

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pScRad52-SpCas9 (pXK-1646)

   Plasmid

   #208823

   Purpose

   CRISPR/Rad52-Cas9 expression vector for Animal gene editing, harboring the ScRad52-SpCas9 and a VEGFA.gRNA expression cassette. Key Construct: hU6-VEGFa.gRNA-CMV-ScRad52-SpCas9.

   Depositor

   Kun Xu

   Article

   Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

   Type

   Empty backbone

   Use

   CRISPR; Cas9

   Tags

   Rad52

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)/AUG-DAP5-3XFLAG

   Plasmid

   #127331

   Purpose

   Express 3xFLAG tagged DAP5 protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   DAP5 (EIF4G2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM7

   Plasmid

   #154164

   Purpose

   mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   mCherry
   

   Expression

   Insect

   Promoter

   IE1 promoter and hr5 enhancer from Autographa cal…

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLminP_Luc2P_RE5

   Plasmid

   #90339

   Purpose

   STAT3 - Stat3 homodimer gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   STAT3

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX-TRE-dCas9-KRAB-MeCP2-BSD

   Plasmid

   #140690

   Purpose

   Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   Cas9m4-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2987

   Plasmid

   #144463

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RORA (RORA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

    Plasmid

    #86828

    Purpose

    Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-puroeGFP

    Plasmid

    #177922

    Purpose

    Constitutively expresses eGFP with puromycin selection

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    eGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CIp10 - mScarlet-I IDT

    Plasmid

    #163120

    Purpose

    Expresses in Candida albicans

    Depositor

    Patrick Van Dijck

    Article

    Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

    Insert

    mScarlet-I IDT
    

    Use

    Candida albicans expression

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV-U6+27-Tornado-F30-TAR Variant-1

    Plasmid

    #129406

    Purpose

    circular TAR Variant-1

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    circular F30-TAR Variant-1
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF0068

    Plasmid

    #142444

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF451 (ZNF451 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF2971

    Plasmid

    #144447

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1H4 (NR1H4 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1 mCherry

    Plasmid

    #128073

    Purpose

    puro exchanged to mCherry

    Depositor

    Oskar Laur

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    no

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBPHLWL-LexA

    Plasmid

    #26258

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    LexA
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV mGAD65(delE1) GFP WPRE SV40pA

    Plasmid

    #177316

    Purpose

    To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

    Depositor

    Hirokazu Hirai

    Article

    Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

    Insert

    mGAD65(delE1) promoter, GABAergic interneurons specific promoter
    

    Use

    AAV

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBiT3.1-C_PALB2:1-1186

    Plasmid

    #211160

    Purpose

    Mammalian expression of full-length human PALB2. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    PALB2:M1-S1186 (PALB2 Human)
    

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSIN-TRE-H2BeGFP-rtTA2

    Plasmid

    #165494

    Purpose

    Doxycycline-inducible all-in-one lentivirus vector to express H2BeGFP fusion protein. System for labelling slow-cycling cells in vivo.

    Depositor

    Hector G. Palmer

    Article

    Puig et al J Clin Invest. 2018 Aug 31;128(9):3887-3

    Insert

    Human histone H2B (H2BC21 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Mutation

    -

    Promoter

    TetO-CMV

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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