pCAG-luciferase
(Plasmid #55764)

• Purpose: luciferase reporter for in vivo bioluminescence imaging
• Depositing Lab: Snorri Thorgeirsson
• Publication: Lee et al Cancer Res. 2010 Nov 1;70(21):8264-9. doi: 10.1158/0008-5472.CAN-10-0749. Epub 2010 Oct 19.

Backbone

• Vector backbone: pGL4.17[luc2/Neo]
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5599
• Total vector size (bp): 7323
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CMV enhancer/chicken beta actin promoter
• Insert Size (bp): 1732
• Promoter: CMV enhancer/chicken beta actin promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (destroyed during cloning)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: RV3 primer
• 3′ sequencing primer: pCAG-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The SalI/EcoRV restriction fragment containing the CMV enhancer/chicken beta actin promoter was cut out of the pCAGEN plasmid (Addgene 11160)
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry