STARR-seq luciferase validation vector_ORI_empty
(Plasmid #99297)

• Purpose: (Empty Backbone) Luciferase validation vector; empty vector without insert to determine baseline
• Depositing Lab: Alexander Stark
• Publication: Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.

Backbone

• Vector backbone: STARR-seq luciferase validation vector_ORI_empty
• Backbone manufacturer: Promega
• Backbone size (bp): 3983
• Vector type: Mammalian Expression, Luciferase ; STARR-seq Luciferase validation vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Resource Information

• Supplemental Documents:
  • STARR-seq luciferase validation vector_ORI_empty (seq_FldkQrWf).gb
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector serves as a control for Luciferase assays. The origin of replication substitutes the core promoter; there is no enhancer cloned into this construct . Please see bioRxiv for preprint article. Posted 17 July 2017. DOI: 10.1101/164590