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pLNHA-C1-HsXRN1
(Plasmid #66596)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66596 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLNHA-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4174
  • Total vector size (bp) 9295
  • Modifications to backbone
    Exchanged the EGFP tag from pEGFP-C1 by a lambdaN-HA-tag
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XRN1
  • Alt name
    SEP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5121
  • GenBank ID
    NM_019001
  • Entrez Gene
    XRN1 (a.k.a. SEP1)
  • Promoter CMV
  • Tag / Fusion Protein
    • lambdaN-HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV for
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLNHA-C1-HsXRN1 was a gift from Elisa Izaurralde (Addgene plasmid # 66596 ; http://n2t.net/addgene:66596 ; RRID:Addgene_66596)
  • For your References section:

    A direct interaction between DCP1 and XRN1 couples mRNA decapping to 5' exonucleolytic degradation. Braun JE, Truffault V, Boland A, Huntzinger E, Chang CT, Haas G, Weichenrieder O, Coles M, Izaurralde E. Nat Struct Mol Biol. 2012 Dec;19(12):1324-31. doi: 10.1038/nsmb.2413. Epub 2012 Nov 11. 10.1038/nsmb.2413 PubMed 23142987