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pcDNA3 Lats1 (Nigg HS189)
(Plasmid #41156)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41156 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1/3x myc-C
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 8800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Lats1
  • Alt name
    LATS, large tumor suppressor, homolog 1
  • Alt name
    WARTS
  • Alt name
    wts
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3300
  • GenBank ID
    NM_001270519.1 NM_004690.3;
  • Entrez Gene
    LATS1 (a.k.a. WARTS, wts)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3xMyc (N terminal on backbone)
    • PreScission Site (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI/BglII (destroyed during cloning)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human LATS1 cDNA from Hideyuki Saya.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human LATS1 cDNA, a kind gift from Hideyuki Saya, was cloned into pcDNA3.1/3x myc-C encoding an amino-terminal 3 x myc tag and verified by sequencing.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 Lats1 (Nigg HS189) was a gift from Erich Nigg (Addgene plasmid # 41156 ; http://n2t.net/addgene:41156 ; RRID:Addgene_41156)
  • For your References section:

    The Ste20-like kinase Mst2 activates the human large tumor suppressor kinase Lats1. Chan EH, Nousiainen M, Chalamalasetty RB, Schafer A, Nigg EA, Sillje HH. Oncogene. 2005 Mar 17;24(12):2076-86. 10.1038/sj.onc.1208445 PubMed 15688006