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pET28a-SpyTagMBP Citations (6)

Originally described in: Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin.
Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Epub 2012 Feb 24.
PubMed Journal

Articles Citing pET28a-SpyTagMBP

Programmable polyproteams built using twin peptide superglues. Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M. Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. PubMed

Associated Plasmids

Engineering a Rugged Nanoscaffold to Enhance Plug-and-Display Vaccination. Bruun TUJ, Andersson AC, Draper SJ, Howarth M. ACS Nano. 2018 Jul 20. doi: 10.1021/acsnano.8b02805. PubMed

Associated Plasmids

Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions. Wieduwild R, Howarth M. Biomaterials. 2018 Oct;180:253-264. doi: 10.1016/j.biomaterials.2018.07.020. Epub 2018 Jul 17. PubMed
Insider information on successful covalent protein coupling with help from SpyBank. Keeble AH, Howarth M. Methods Enzymol. 2019;617:443-461. doi: 10.1016/bs.mie.2018.12.010. Epub 2019 Jan 25. PubMed
Structures of a non-ribosomal peptide synthetase condensation domain suggest the basis of substrate selectivity. Izore T, Candace Ho YT, Kaczmarski JA, Gavriilidou A, Chow KH, Steer DL, Goode RJA, Schittenhelm RB, Tailhades J, Tosin M, Challis GL, Krenske EH, Ziemert N, Jackson CJ, Cryle MJ. Nat Commun. 2021 May 4;12(1):2511. doi: 10.1038/s41467-021-22623-0. PubMed
N-Terminal Modification of Gly-His-Tagged Proteins with Azidogluconolactone. Brune KD, Lieknina I, Sutov G, Morris AR, Jovicevic D, Kalnins G, Kazaks A, Kluga R, Kastaljana S, Zajakina A, Jansons J, Skrastina D, Spunde K, Cohen AA, Bjorkman PJ, Morris HR, Suna E, Tars K. Chembiochem. 2021 Nov 16;22(22):3199-3207. doi: 10.1002/cbic.202100381. Epub 2021 Oct 6. PubMed

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