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human EB1 fused to monomeric RFP
(Plasmid #39323)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39323 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 3982
  • Total vector size (bp) 5461
  • Modifications to backbone
    GFP cut out to swap in mRFP as a BamH1 - Not1 fragment. The mRFP is a PCR product from R Tsien's mRFP plasmid.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    EB1
  • Alt name
    MAPRE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    804
  • Entrez Gene
    MAPRE1 (a.k.a. EB1)
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer mCherry-R
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mRFP
  • Alt name
    monomeric RFP
  • Species
    Synthetic
  • Insert Size (bp)
    675

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mRFP from Roger Tsien
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct makes very nice comets on growing microtubule tips; mRFP is early generation from ~2002, excitation max 584 nm and emission max 607 nm.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human EB1 fused to monomeric RFP was a gift from Tim Mitchison & Jennifer Tirnauer (Addgene plasmid # 39323 ; http://n2t.net/addgene:39323 ; RRID:Addgene_39323)