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Purposeexpresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTs
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Depositing Lab
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Depositing OrganizationUniversity of Wisconsin, Madison
Located in the United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 50708 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 50708-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 50708-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonetdTomato-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5443
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEB3
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Alt nameMAPRE3
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SpeciesH. sapiens (human)
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Entrez GeneMAPRE3 (a.k.a. EB3, EBF3, EBF3-S, RP3)
- Promoter CMV
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Tag
/ Fusion Protein
- tdTomato (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sal1 (not destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
We received the EB3 construct from Niels Galjart (Erasmus U, Netherlands) originally and replaced GFP with tdTomato.
There is a Sal I site at the 5' end of EB3 and a Not I site at the 3' end of tdTomato.
DNA (Catalog # 50708-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 50708-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EB3-tdTomato was a gift from Erik Dent (Addgene plasmid # 50708 ; http://n2t.net/addgene:50708 ; RRID:Addgene_50708) -
For your References section:
Synaptic regulation of microtubule dynamics in dendritic spines by calcium, F-actin, and drebrin. Merriam EB, Millette M, Lumbard DC, Saengsawang W, Fothergill T, Hu X, Ferhat L, Dent EW. J Neurosci. 2013 Oct 16;33(42):16471-82. doi: 10.1523/JNEUROSCI.0661-13.2013. 10.1523/JNEUROSCI.0661-13.2013 PubMed 24133252
