EB3-tdTomato
(Plasmid #50708)

• Purpose: expresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTs
• Depositing Lab: Erik Dent
• Publication: Merriam et al J Neurosci. 2013 Oct 16;33(42):16471-82. doi: 10.1523/JNEUROSCI.0661-13.2013.

Backbone

• Vector backbone: tdTomato-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5443
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EB3
• Alt name: MAPRE3
• Species: H. sapiens (human)
• Entrez Gene: MAPRE3 (a.k.a. EB3, EBF3, EBF3-S, RP3)
• Promoter: CMV
• Tag / Fusion Protein:
  • tdTomato (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (not destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We received the EB3 construct from Niels Galjart (Erasmus U, Netherlands) originally and replaced GFP with tdTomato.
There is a Sal I site at the 5' end of EB3 and a Not I site at the 3' end of tdTomato.