164,223 results

1. pAAV FLEX Synaptophysin GFP

   Plasmid

   #137188

   Purpose

   Cre-dependent expression of synaptophysin-GFP

   Depositor

   Matthew Nolan

   Article

   Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

   Insert

   Synaptophysin GFP (Syp Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29-bdSUMO-Anti-ALFA nanobody

   Plasmid

   #212311

   Purpose

   Expression of anti-ALFA nanobody

   Depositor

   Arthur Laganowsky

   Article

   Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

   Insert

   anti-ALFA nanobody
   

   Use

   Tags

   bdSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. tetO.Nfib.Hygro

   Plasmid

   #117271

   Purpose

   Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

   Depositor

   Henrik Ahlenius

   Article

   Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

   Insert

   Nfib (Nfib Mouse)
   

   Use

   Lentiviral

   Tags

   IRES and hygromycin resistant cassette (C termina…

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FlucSM-HA-GFP11-N1

   Plasmid

   #91955

   Purpose

   Expresses aggregation-prone FlucSM-HA-GFP11 in mammalian cells

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   FlucSM-HA-GFP11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R188Q

   Promoter

   CMV

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF2241

   Plasmid

   #141985

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GATA3 (GATA3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Intron-Tagging-EGFP-Frame0-Parental-Minicircle

   Plasmid

   #216124

   Purpose

   Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57kan-T7-gRNA-U6 V2

   Plasmid

   #115520

   Purpose

   To construct multiple sgRNA expression vector for CRISPR

   Depositor

   Xingxu Huang

   Insert

   sgRNA scaffolf-U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDualEX-SMN2

   Plasmid

   #214431

   Purpose

   Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN2 EXON 7 (SMN2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDIV643

   Plasmid

   #226182

   Purpose

   Expresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

   Insert

   uidA marker flanked with targeting regions to KU70 gene in K.phaffii
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TEF1p

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Syn-Myr_mCherry-2A-eGFP

    Plasmid

    #194877

    Purpose

    Ratiometric Myr/Palm_mCherry control probe

    Depositor

    Peter Scheiffele

    Article

    Czernecki et al bioRxiv 2022.11.16.516849

    Insert

    Myristoylated/Palmitoylated_mCherry-T2A-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57.1_AOX-FLAG

    Plasmid

    #177984

    Purpose

    All-in-one dox-inducible expression of Alternative Oxidase

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    AOX
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Codon optimized cDNA for expression in human cells

    Promoter

    TRE

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tet-inducible Luciferase reporter

    Plasmid

    #64127

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetOx13-CMV

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. hCas9-VPR

    Plasmid

    #68497

    Purpose

    nuclease competent SP-Cas9 fused to VPR

    Depositor

    George Church

    Article

    Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

    Insert

    SP-Cas9-VPR
    

    Use

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

    Plasmid

    #113888

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMVInt

    Plasmid

    #18935

    Depositor

    Michele Calos

    Article

    Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. EdTP

    Plasmid

    #24311

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    31 first amino acids of hTcf4 have been deleted.

    Promoter

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET_RTX(exo-)-6xHis

    Plasmid

    #145028

    Purpose

    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX(exo-)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N210D

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLifeAct-mTurquoise2

    Plasmid

    #36201

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAS9-mCherry-Frame +1

    Plasmid

    #66940

    Purpose

    CRISPaint frame selector +1

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 TAK1/F

    Plasmid

    #44161

    Depositor

    Xin Lin

    Article

    Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

    Insert

    Tak1 (Map3k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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