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pmScarlet_Giantin_C1

Plasmid

#85048

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pmScarlet_peroxisome_C1

Plasmid

#85063

Purpose

In vivo visualization of the peroxisome (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Peroxisomal targeting sequence

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLifeAct_mScarlet-i_N1

Plasmid

#85056

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTDpelB-NTwinStrep

Plasmid

#45940

Purpose

use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

6xHis, PelB, and Twin-Strep

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_A2_F

Plasmid

#237351

Purpose

Expression plasmid for RSV A2 F

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV A2 F human codon optimized from Genbank Accesion QGW56794.1

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDV05.2_pEF1a-iRFP720_secreted

Plasmid

#200409

Purpose

Expresses a secreted version of iRFP720 constitutively in mammalian cells

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

iRFP720 (secreted)

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe RFP1-KRT5 hygro

Plasmid

#58493

Purpose

mammalian expression of mRFP1-tagged human KRT5

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

KRT5 (KRT5 Human)

Tags

mRFP1

Expression

Mammalian

Available Since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
pJL1-sfGFP

Plasmid

#102634

Purpose

In vitro expression of sfGFP from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

sfGFP

Tags

strep tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pHoss1

Plasmid

#63158

Purpose

Suicide plasmid for efficient gene mutation in Gram-positive bacteria

Depositor

Attila Karsi

Article

Abdelhamed et al Plasmid. 2015 May 31;81:1-8. doi: 10.101

Insert

anti-secY-Pxyl/tetO-tetR

Use

Suicide plasmid for gram-positive bacteria

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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V406 4HA-CKIe(K38R)

Plasmid

#13725

Depositor

David Virshup

Article

Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

Insert

casein kinase 1 epsilon (CSNK1E Human)

Tags

4 HA

Expression

Mammalian

Mutation

kinase-inactive due to K38R mutation

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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phage-cmv-cfp-24xpp7

Plasmid

#40652

Depositor

Robert Singer

Article

Wu et al Biophys J. 2012 Jun 20;102(12):2936-44.

Insert

CFP-24xPP7

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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3XFLAG-VP64-SadCas9-NLS-VP64

Plasmid

#135338

Purpose

3xFLAG 2xVP64 SadCas9 for single guide cloning

Depositor

Ronald Cohn

Article

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Insert

SadCas9

Tags

3xFLAG-VP64-SV40 NLS and NLS-VP64

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_Hinge Notch SNIPR (Notch2 JMD)

Plasmid

#188375

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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DT40

Plasmid

#80415

Purpose

Expresses 40 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 40 interrupted CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Mm.cargo(Cre)

Plasmid

#174862

Purpose

Expresses mouse Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by MmPeg10 UTRs

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pM162

Plasmid

#173221

Purpose

Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Inserts

ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
MCP-mCherry-GFP11-GB1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-ccdB-3xFLAG-V5

Plasmid

#87071

Purpose

Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-M-GFP

Plasmid

#11952

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-M-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Methionine …

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.LSL.tdTomato

Plasmid

#100048

Purpose

AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdtomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Promoter

None

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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