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pCAG-HIF1 alpha
(Plasmid #21101)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21101 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAGEN
  • Backbone manufacturer
    Addgene Plasmid 11160
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HYPOXIA INDUCIBLE FACTOR 1ALPHA
  • Alt name
    HIF1A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2400
  • Entrez Gene
    HIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NOT1 (not destroyed)
  • 3′ cloning site NOT1 (not destroyed)
  • 5′ sequencing primer pCAG-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    K.W. Kim, Seoul National University
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Compared to GenBank reference sequence NP_001521.1 the HIF1 alpha insert in this plasmid contains C217Y & A279D mutations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HIF1 alpha was a gift from Connie Cepko (Addgene plasmid # 21101 ; http://n2t.net/addgene:21101 ; RRID:Addgene_21101)
  • For your References section:

    HDAC4 regulates neuronal survival in normal and diseased retinas. Chen B, Cepko CL. Science. 2009 Jan 9. 323(5911):256-9. 10.1126/science.1166226 PubMed 19131628