|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21101||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene Plasmid 11160
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameHYPOXIA INDUCIBLE FACTOR 1ALPHA
SpeciesH. sapiens (human)
Insert Size (bp)2400
Entrez GeneHIF1A (a.k.a. HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78)
- Cloning method Restriction Enzyme
- 5′ cloning site NOT1 (not destroyed)
- 3′ cloning site NOT1 (not destroyed)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Compared to GenBank reference sequence NP_001521.1 the HIF1 alpha insert in this plasmid contains C217Y & A279D mutations.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-HIF1 alpha was a gift from Connie Cepko (Addgene plasmid # 21101 ; http://n2t.net/addgene:21101 ; RRID:Addgene_21101)
For your References section:HDAC4 regulates neuronal survival in normal and diseased retinas. Chen B, Cepko CL. Science. 2009 Jan 9. 323(5911):256-9. 10.1126/science.1166226 PubMed 19131628