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pVax1-hVEGF165
(Plasmid #74466)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74466 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pVax1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 3578
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    vascular endothelial growth factor 165
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    578
  • Entrez Gene
    VEGFA (a.k.a. MVCD1, VEGF, VPF)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BspI/HindIII (destroyed during cloning)
  • 3′ cloning site NheI/XbaI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A portion of this plasmid was derived from plasmid pBLASThVEGF, Invivogen
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVax1-hVEGF165 was a gift from Loree Heller (Addgene plasmid # 74466 ; http://n2t.net/addgene:74466 ; RRID:Addgene_74466)
  • For your References section:

    Electroporation-mediated delivery of a naked DNA plasmid expressing VEGF to the porcine heart enhances protein expression. Marshall WG Jr, Boone BA, Burgos JD, Gografe SI, Baldwin MK, Danielson ML, Larson MJ, Caretto DR, Cruz Y, Ferraro B, Heller LC, Ugen KE, Jaroszeski MJ, Heller R. Gene Ther. 2010 Mar;17(3):419-23. doi: 10.1038/gt.2009.153. Epub 2009 Dec 3. 10.1038/gt.2009.153 PubMed 19956270