Purposeexpression of hVEGF165 in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74466||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 3578
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namevascular endothelial growth factor 165
SpeciesH. sapiens (human)
Insert Size (bp)578
Entrez GeneVEGFA (a.k.a. MVCD1, VEGF, VPF)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BspI/HindIII (destroyed during cloning)
- 3′ cloning site NheI/XbaI (destroyed during cloning)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pVax1-hVEGF165 was a gift from Loree Heller (Addgene plasmid # 74466 ; http://n2t.net/addgene:74466 ; RRID:Addgene_74466)
For your References section:Electroporation-mediated delivery of a naked DNA plasmid expressing VEGF to the porcine heart enhances protein expression. Marshall WG Jr, Boone BA, Burgos JD, Gografe SI, Baldwin MK, Danielson ML, Larson MJ, Caretto DR, Cruz Y, Ferraro B, Heller LC, Ugen KE, Jaroszeski MJ, Heller R. Gene Ther. 2010 Mar;17(3):419-23. doi: 10.1038/gt.2009.153. Epub 2009 Dec 3. 10.1038/gt.2009.153 PubMed 19956270