|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15777||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
CAG promoter is a more efficient promoter than CMV, see "Efficient selection for high-expression transfectants with a novel eukaryotic vector", Gene. 1991 Dec 15;108(2):193-9.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-DsRed2 was a gift from Edward Callaway (Addgene plasmid # 15777 ; http://n2t.net/addgene:15777 ; RRID:Addgene_15777)
For your References section:Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons. Wickersham IR, Lyon DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, Young JA, Callaway EM. Neuron. 2007 Mar 1. 53(5):639-47. 10.1016/j.neuron.2007.01.033 PubMed 17329205