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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 26721 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
This item is currently unavailable outside the US without additional regulatory approval.
A non-refundable shipping export licensing fee of $85 is required to cover Addgene’s additional processing costs.
Backbone
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Vector backbonepCAG-venusYFP
- Backbone size w/o insert (bp) 3520
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsrGI (not destroyed)
- 3′ cloning site StuI (destroyed during cloning)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the Addgene sequencing result shows a CMV promoter is present. A CAG promoter was not identified as the plasmid name implies.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-YTB was a gift from Edward Callaway (Addgene plasmid # 26721 ; http://n2t.net/addgene:26721 ; RRID:Addgene_26721) -
For your References section:
Targeting single neuronal networks for gene expression and cell labeling in vivo. Marshel JH, Mori T, Nielsen KJ, Callaway EM. Neuron. 2010 Aug 26. 67(4):562-74. 10.1016/j.neuron.2010.08.001 PubMed 20797534
Map uploaded by the depositor.
