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pUC gRNA Shuttle Citations (8)

Originally described in: Targeted genome modifications in soybean with CRISPR/Cas9.
Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
PubMed Journal

Articles Citing pUC gRNA Shuttle

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots. Jacobs TB, Martin GB. J Vis Exp. 2016 Apr 30;(110). doi: 10.3791/53843. PubMed
Mutagenesis of FAD2 genes in peanut with CRISPR/Cas9 based gene editing. Yuan M, Zhu J, Gong L, He L, Lee C, Han S, Chen C, He G. BMC Biotechnol. 2019 Apr 29;19(1):24. doi: 10.1186/s12896-019-0516-8. PubMed
t-SNAREs bind the Rhg1 alpha-SNAP and mediate soybean cyst nematode resistance. Dong J, Zielinski RE, Hudson ME. Plant J. 2020 Jul 9. doi: 10.1111/tpj.14923. PubMed
The application of CRISPR/Cas9 in hairy roots to explore the functions of AhNFR1 and AhNFR5 genes during peanut nodulation. Shu H, Luo Z, Peng Z, Wang J. BMC Plant Biol. 2020 Sep 7;20(1):417. doi: 10.1186/s12870-020-02614-x. PubMed
Tailoring poplar lignin without yield penalty by combining a null and haploinsufficient CINNAMOYL-CoA REDUCTASE2 allele. De Meester B, Madariaga Calderon B, de Vries L, Pollier J, Goeminne G, Van Doorsselaere J, Chen M, Ralph J, Vanholme R, Boerjan W. Nat Commun. 2020 Oct 6;11(1):5020. doi: 10.1038/s41467-020-18822-w. PubMed
Multiplex knockout of trichome-regulating MYB duplicates in hybrid poplar using a single gRNA. Bewg WP, Harding SA, Engle NL, Vaidya BN, Zhou R, Reeves J, Horn TW, Joshee N, Jenkins JW, Shu S, Barry KW, Yoshinaga Y, Grimwood J, Schmitz RJ, Schmutz J, Tschaplinski TJ, Tsai CJ. Plant Physiol. 2022 Jun 1;189(2):516-526. doi: 10.1093/plphys/kiac128. PubMed
Multiplex Editing of the Nucleoredoxin1 Tandem Array in Poplar: From Small Indels to Translocations and Complex Inversions. Chen YH, Sharma S, Bewg WP, Xue LJ, Gizelbach CR, Tsai CJ. CRISPR J. 2023 Aug;6(4):339-349. doi: 10.1089/crispr.2022.0096. Epub 2023 Jun 12. PubMed
Knockout of floral and meiosis genes using CRISPR/Cas9 produces male-sterility in Eucalyptus without impacts on vegetative growth. Nagle MF, Nahata SS, Zahl B, Nino de Rivera A, Tacker XV, Elorriaga E, Ma C, Goralogia GS, Klocko AL, Gordon M, Joshi S, Strauss SH. Plant Direct. 2023 Jul 14;7(7):e507. doi: 10.1002/pld3.507. eCollection 2023 Jul. PubMed

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