164,220 results
-
Plasmid#231988PurposeKnockout non-targeting controlDepositorInsertsgRNA with Cas9 and hygromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA2-Fab-HC-Halo
Plasmid#230061PurposeExpresses heavy chain Fab fragment of anti-GluA2 fused to HaloTag. Can be used with light chain to produce Fab in HEK cells.DepositorInsertAnti-GluA2 Fab heavy chain fragment
UseTagsHaloTagExpressionMammalianMutationPromoterChicken beta actin (CAG)Available SinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
TFORF0105
Plasmid#142535PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) EGFP-Rap1A(WT)
Plasmid#224276PurposeCell transfection and expression of Rap1ADepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221076PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertGGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221077PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertCCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221079PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertTTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_AF
Plasmid#217598PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_p15A_AF
Plasmid#217588PurposeDestination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Bcl-2-ΔBH4
Plasmid#220300Purposemammalian expression of Bcl-2-ΔBH4 tagged with GFPDepositorInsertBcl2 (BCL2 Human)
UseTagsEmerald GFPExpressionMammalianMutationBH4 deletedPromoterCMVAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD061
Plasmid#212915PurposeEncodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDO068
Plasmid#198788PurposeDrives specific expression in the BAG neuronsDepositorInsertPgcy-33-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2ss-U6-sgHTT1-7sk-sgCas9
Plasmid#190900PurposeAAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAsDepositorAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIGLEC8-COMP5AP-AviTag-9xHis
Plasmid#157356PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertSIGLEC8 (SIGLEC8 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-SIGLEC15-COMP5AP-AviTag-9xHis
Plasmid#157262PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertSIGLEC15 (SIGLEC15 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV Myc-MBP
Plasmid#175103PurposeLentiviral expression of Myc-tagged MBPDepositorInsertMBP
UseLentiviralTagsMycExpressionMammalianMutationPromoterAvailable SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK027
Plasmid#176731PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Saccharomyces cerevisiae (receiver part).DepositorArticleInsertste2Sc
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK002
Plasmid#176706PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter FUS1 of S. cerevisiae (response part)DepositorArticleInsertP-FUS1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)
Plasmid#171100PurposeCRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.DepositorAvailable SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVpuro-CMV-N-3xFLAG
Plasmid#123223PurposeN-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.DepositorTypeEmpty backboneUseLentiviral; Gateway destinationTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only