166,137 results

1. pT2K-CAGGS-T2TP

   Plasmid

   #114725

   Purpose

   expression in mammalian cells

   Depositor

   Martine Roussel

   Article

   Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

   Insert

   Tol2 transposase
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST53-LRRK2-G2019S

   Plasmid

   #25045

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Glycine 2019 mutated to Serine

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCB6 ZO1myc

   Plasmid

   #30317

   Depositor

   James Anderson, Alan Fanning

   Article

   Fanning et al J Biol Chem. 1998 Nov 6. 273(45):29745-5

   Insert

   Zonula Occludens-1 (TJP1 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-FLEX-SaCas9-U6-sgGrin1

   Plasmid

   #124852

   Purpose

   Mutagenesis of Grin1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin1 (Grin1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. BFP-Dcp1a

   Plasmid

   #153973

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1a

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA decapping enzyme 1a (DCP1A Human)
   

   Tags

   mTagBFP-c1

   Expression

   Mammalian

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pP3-GFP

   Plasmid

   #53436

   Purpose

   Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   P3 promoter
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   P3

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-LMNA-gRNA1

   Plasmid

   #122507

   Purpose

   expresses WT spCas9 and a chimeric gRNA targeting human LMNA

   Depositor

   Graham Dellaire

   Article

   Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

   Insert

   LMNA-gRNA1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Beclin1

   Plasmid

   #21150

   Depositor

   Junying Yuan

   Article

   Shibata et al J Biol Chem. 2006 May 19. 281(20):14474-

   Insert

   PcDNA3-Beclin1 (BECN1 Human)
   

   Expression

   Mammalian

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-opEBNA2-IRES-GFP

   Plasmid

   #220354

   Purpose

   Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Tags

   3 x HA tag

   Expression

   Mammalian

   Mutation

   codon-optimised for expression in mammalian cells

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-puro-CMV-CC3AI

    Plasmid

    #78909

    Purpose

    Indicator of caspase-3-like protease activity

    Depositor

    Binghui Li

    Article

    Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

    Insert

    CC3AI
    

    Use

    Lentiviral

    Tags

    HA and Myc

    Mutation

    S320A, deletion of AA1-200

    Promoter

    CMV

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

11. TFORF0630

    Plasmid

    #142668

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1I2 (NR1I2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-alphasynuclein-A53T

    Plasmid

    #40823

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    A53T (aggregates more rapidly than the wild-type …

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-hPAX7 (#348)

    Plasmid

    #78337

    Purpose

    Overexpression of human PAX7 ORF with a N-terminal FLAG tag

    Depositor

    Matthew Alexander, Louis Kunkel

    Insert

    PAX7 (PAX7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

14. M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1

    Plasmid

    #122147

    Purpose

    Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Lentiviral

    Tags

    NLS-iLID-GFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4.23_Mmp11

    Plasmid

    #209520

    Purpose

    Expresses luciferase under control of Mmp11 promoter in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

    Insert

    Mmp11 promoter (Mmp11 Mouse)
    

    Use

    Luciferase

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-6xHis-GFP(+30)

    Plasmid

    #199164

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+30)
    

    Tags

    MGHHHHHHGGA

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…

    Promoter

    T7

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRha-ABE8e-SpCas9-NG

    Plasmid

    #201190

    Purpose

    Expresses the base editor ABE8e-SpCas9-NG in bacterial cells

    Depositor

    Jonathan Yen

    Article

    Mayuranathan et al Nat Genet. 2023 Jul;55(7):1210-1220. doi

    Insert

    ABE8e-SpCas9-NG
    

    Tags

    8xHIS and BP-NLS

    Expression

    Bacterial

    Promoter

    pRhaBAD

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-TBK1-C-FLAG-HA

    Plasmid

    #131792

    Purpose

    Lentiviral vector expressing WT TBK1 containing STOP codon

    Depositor

    Alicia Pickrell

    Article

    Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

    Insert

    TBK1 WT (TBK1 Human)
    

    Use

    Lentiviral

    Tags

    STOP codon

    Expression

    Mammalian

    Mutation

    WT full length

    Promoter

    CMV

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Plasmid

    #179105

    Purpose

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #180280

    Purpose

    The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (backbone) and BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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