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Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design.
Smith JD, Suresh S, Schlecht U, Wu M, Wagih O, Peltz G, Davis RW, Steinmetz LM, Parts L, St Onge RP
Genome Biol. 2016 Mar 8;17(1):45. doi: 10.1186/s13059-016-0900-9.
PubMed

Plasmids from Article

ID Plasmid  
73796pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA
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