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DisCoTune: versatile auxiliary plasmids for the production of disulphide-containing proteins and peptides in the E. coli T7 system.
Bertelsen AB, Hackney CM, Bayer CN, Kjelgaard LD, Rennig M, Christensen B, Sorensen ES, Safavi-Hemami H, Wulff T, Ellgaard L, Norholm MHH
Microb Biotechnol. 2021 Nov;14(6):2566-2580. doi: 10.1111/1751-7915.13895. Epub 2021 Aug 18. PubMed Article

Plasmids from Article

ID Plasmid Purpose
173713pDisCoTunePromotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

Antibodies from Article