169,327 results
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Plasmid#85102PurposeP450 BM3 variant (A74G/C773S/C810S)DepositorInsertP450 BM3
UseCloning vectorMutationA74G/C773S/C810SAvailable SinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
VT028327-FRT10-STOP-FRT10-GAL4
Plasmid#232829PurposeEnhancer VT028327 driven GAL4, gated by Sparse.FRT10DepositorInsertsExpressionInsectAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS59
Plasmid#215680PurposeCas9 + guide plasmid targeting ChrI split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
UseCRISPRExpressionWormAvailable SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LacI
Plasmid#46394PurposeOverproduction of LacIDepositorInsertLacI
Tags6xHIS tagExpressionBacterialMutationsilent T to C mutation at position 19Promoterarabinose promoterAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:GtACR2-tdTomato
Plasmid#124236PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin GtACR2 fused to the red fluorescent protein tdTomatoDepositorInsertGtACR2-tdTomato
UseZebrafish expressionTagstdTomatoPromoter14xUAS-E1bAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_SPTLC1_WT
Plasmid#181920Purpose3rd generation, Inducible lentiviral plasmid for expression of human wildtype SPTLC1DepositorInsertHuman serine palmitoyltransferase, long chain base subunit 1 (SPTLC1), (SPTLC1 Human)
UseLentiviral; Mammalian expression, , doxycycline i…ExpressionMammalianPromotertight TRE promoterAvailable SinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
PM-RA
Plasmid#211705PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (RA) fused with plasma membrane (PM) targetting motif of Lck in mammalian cellsDepositorAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_ZIKV_PrME
Plasmid#207316PurposeMammalian expression of Zika virus PrME proteinDepositorInsertZIKV_PrME (POLY Zika virus)
ExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-UBE4B
Plasmid#113499PurposeExpression of human UBE4B with N-terminal GFP tagDepositorAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmiRFP680-T2A-HO1
Plasmid#197244PurposeExpresses the protein of wmiRFP680-T2A-HO1 in neurons of C eleganDepositorInsertwmiRFP680-T2A-HO1
ExpressionWormAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA
Plasmid#109314PurposePlasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNADepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable SinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSIQ
Plasmid#216238PurposeAll-in One IQ transgene switch, empty vectorDepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianAvailable SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
Thy1 promoter construct
Plasmid#20736DepositorInsertThy1 promoter (Thy1 Mouse)
ExpressionMammalianAvailable SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pS548∙CsR
Plasmid#197859PurposeExpresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteriaDepositorInsertgRNA scaffold - Tc resistance cassette - incP oriT
UseCRISPR and Synthetic BiologyPromoterPEM7Available SinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF3015
Plasmid#144491PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21b FAST-PETase
Plasmid#225760PurposeExpresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tagDepositorInsertFAST-PETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-GRM3 WT
Plasmid#31798DepositorAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
B-GRAM-H
Plasmid#211706PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H) in mammalian cellsDepositorInsertB-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
TagsddFP-B3ExpressionMammalianMutationChanged Glycine 187 to LeucinePromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
GolgiCFP
Plasmid#14873DepositorInsertCFP
TagseNOS(1-35)ExpressionMammalianAvailable SinceAug. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
DRH296: FCK-Optopatch2
Plasmid#51694PurposeCoexpression plasmid of QuasAr2 and CheRiff for all-optical electrophysiologyDepositorInsertOptopatch2
UseLentiviralExpressionMammalianPromoterCaMKIIaAvailable SinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only