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PurposeExpresses anti-mouse nanobody fused with Tn5
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Depositing Lab
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Publication
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183637 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-29b
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameanti mouse secondary nanobody fused to Tn5
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Alt namems-Tn5
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SpeciesE. coli
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Insert Size (bp)2088
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Tag
/ Fusion Protein
- anti-mouse nanobody
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe construct was ordered as a synthetic gene and cloned at Twist Bioscience
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
psfaMs-Tn5 was a gift from Goncalo Castelo-Branco (Addgene plasmid # 183637 ; http://n2t.net/addgene:183637 ; RRID:Addgene_183637) -
For your References section:
Multimodal chromatin profiling using nanobody-based single-cell CUT&Tag. Bartosovic M, Castelo-Branco G. bioRxiv April 08, 2022 10.1101/2022.03.08.483459
