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ER-mRFP
(Plasmid #62236)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62236 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 62236-D50 50 μg of DNA in Tris buffer $495
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DNA 62236-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    clontech C1
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4800
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ER-mRFP
  • Species
    Synthetic
  • Insert Size (bp)
    801
  • Promoter cmv
  • Tag / Fusion Protein
    • mRFP1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer tggtttagtgaaccgt
  • 3′ sequencing primer GTTCAGGGGGAGGTGTG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Red localization marker for mammalian endoplasmic reticulum.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ER-mRFP was a gift from Erik Snapp (Addgene plasmid # 62236 ; http://n2t.net/addgene:62236 ; RRID:Addgene_62236)

  • For your References section:

    Monitoring chaperone engagement of substrates in the endoplasmic reticulum of live cells. Snapp EL, Sharma A, Lippincott-Schwartz J, Hegde RS. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6536-41. Epub 2006 Apr 14. 10.1073/pnas.0510657103 PubMed 16617114
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