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pCAG-T3-hCAS-pA Citations (19)

Originally described in: Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease.
Fujii W, Kawasaki K, Sugiura K, Naito K Nucleic Acids Res. 2013 Aug 30.
PubMed Journal

Articles Citing pCAG-T3-hCAS-pA

Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system. Fujii W, Onuma A, Sugiura K, Naito K. Biochem Biophys Res Commun. 2014 Jan 31. pii: S0006-291X(14)00176-4. doi: 10.1016/j.bbrc.2014.01.141. PubMed

Associated Plasmids

A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs. Romanienko PJ, Giacalone J, Ingenito J, Wang Y, Isaka M, Johnson T, You Y, Mark WH. PLoS One. 2016 Feb 5;11(2):e0148362. doi: 10.1371/journal.pone.0148362. eCollection 2016. PubMed

Associated Plasmids

Efficient generation of FVII gene knockout mice using CRISPR/Cas9 nuclease and truncated guided RNAs. An L, Hu Y, Chang S, Zhu X, Ling P, Zhang F, Liu J, Liu Y, Chen Y, Yang L, Presicce GA, Du F. Sci Rep. 2016 May 3;6:25199. doi: 10.1038/srep25199. PubMed
Zygote-mediated generation of genome-modified mice using Streptococcus thermophilus 1-derived CRISPR/Cas system. Fujii W, Kakuta S, Yoshioka S, Kyuwa S, Sugiura K, Naito K. Biochem Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30988-3. doi: 10.1016/j.bbrc.2016.06.070. PubMed

Associated Plasmids

A critical role of solute carrier 22a14 in sperm motility and male fertility in mice. Maruyama SY, Ito M, Ikami Y, Okitsu Y, Ito C, Toshimori K, Fujii W, Yogo K. Sci Rep. 2016 Nov 4;6:36468. doi: 10.1038/srep36468. PubMed
A Novel Mouse Model of iNKT Cell-deficiency Generated by CRISPR/Cas9 Reveals a Pathogenic Role of iNKT Cells in Metabolic Disease. Ren Y, Sekine-Kondo E, Shibata R, Kato-Itoh M, Umino A, Yanagida A, Satoh M, Inoue K, Yamaguchi T, Mochida K, Nakae S, Van Kaer L, Iwabuchi K, Nakauchi H, Watarai H. Sci Rep. 2017 Oct 6;7(1):12765. doi: 10.1038/s41598-017-12475-4. PubMed
Efficient Generation of Genome-Modified Mice Using Campylobacter jejuni-Derived CRISPR/Cas. Fujii W, Ikeda A, Sugiura K, Naito K. Int J Mol Sci. 2017 Oct 31;18(11). pii: ijms18112286. doi: 10.3390/ijms18112286. PubMed

Associated Plasmids

Generation of App knock-in mice reveals deletion mutations protective against Alzheimer's disease-like pathology. Nagata K, Takahashi M, Matsuba Y, Okuyama-Uchimura F, Sato K, Hashimoto S, Saito T, Saido TC. Nat Commun. 2018 May 4;9(1):1800. doi: 10.1038/s41467-018-04238-0. PubMed
APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. Signes A, Cerutti R, Dickson AS, Beninca C, Hinchy EC, Ghezzi D, Carrozzo R, Bertini E, Murphy MP, Nathan JA, Viscomi C, Fernandez-Vizarra E, Zeviani M. EMBO Mol Med. 2019 Jan;11(1). pii: emmm.201809582. doi: 10.15252/emmm.201809582. PubMed
Genome Editing in Mouse Embryos with CRISPR/Cas9. Scott GJ, Gruzdev A. Methods Mol Biol. 2019;1960:23-40. doi: 10.1007/978-1-4939-9167-9_2. PubMed
Generation of genetically modified mice using SpCas9-NG engineered nuclease. Fujii W, Ito H, Kanke T, Ikeda A, Sugiura K, Naito K. Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.1038/s41598-019-49394-5. PubMed

Associated Plasmids

High-fidelity endonuclease variant HypaCas9 facilitates accurate allele-specific gene modification in mouse zygotes. Ikeda A, Fujii W, Sugiura K, Naito K. Commun Biol. 2019 Oct 10;2:371. doi: 10.1038/s42003-019-0627-8. eCollection 2019. PubMed

Associated Plasmids

Nuclear accumulation of pyruvate dehydrogenase alpha 1 promotes histone acetylation and is essential for zygotic genome activation in porcine embryos. Zhou W, Niu YJ, Nie ZW, Kim JY, Xu YN, Yan CG, Cui XS. Biochim Biophys Acta Mol Cell Res. 2020 Apr;1867(4):118648. doi: 10.1016/j.bbamcr.2020.118648. Epub 2020 Jan 11. PubMed
Nfkb2 variants reveal a p100-degradation threshold that defines autoimmune susceptibility. Wirasinha RC, Davies AR, Srivastava M, Sheridan JM, Sng XYX, Delmonte OM, Dobbs K, Loh KL, Miosge LA, Lee CE, Chand R, Chan A, Yap JY, Keller MD, Chen K, Rossjohn J, La Gruta NL, Vinuesa CG, Reid HH, Lionakis MS, Notarangelo LD, Gray DHD, Goodnow CC, Cook MC, Daley SR. J Exp Med. 2021 Feb 1;218(2). pii: 211502. doi: 10.1084/jem.20200476. PubMed
Dlec1 is required for spermatogenesis and male fertility in mice. Okitsu Y, Nagano M, Yamagata T, Ito C, Toshimori K, Dohra H, Fujii W, Yogo K. Sci Rep. 2020 Nov 3;10(1):18883. doi: 10.1038/s41598-020-75957-y. PubMed
Somatostatin-evoked Abeta catabolism in the brain: Mechanistic involvement of alpha-endosulfine-KATP channel pathway. Watamura N, Kakiya N, Nilsson P, Tsubuki S, Kamano N, Takahashi M, Hashimoto S, Sasaguri H, Saito T, Saido TC. Mol Psychiatry. 2021 Nov 4. pii: 10.1038/s41380-021-01368-8. doi: 10.1038/s41380-021-01368-8. PubMed
Efficient mutagenesis targeting the IFNAR1 gene in mice using a combination of Cas9 protein and dual gRNAs. Liu Z, Chen Z, Di J, Wang Z, Zhang Y, Li J, An L, Presicce GA, Liu L, Du F. Am J Transl Res. 2021 Oct 15;13(10):12094-12106. eCollection 2021. PubMed
CRISPR/Cas9-mediated TGFbetaRII disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells in vitro. Alishah K, Birtel M, Masoumi E, Jafarzadeh L, Mirzaee HR, Hadjati J, Voss RH, Diken M, Asad S. J Transl Med. 2021 Nov 27;19(1):482. doi: 10.1186/s12967-021-03146-0. PubMed
Leishmania infection-induced multinucleated giant cell formation via upregulation of ATP6V0D2 expression. Hong J, Sanjoba C, Fujii W, Yamagishi J, Goto Y. Front Cell Infect Microbiol. 2022 Sep 23;12:953785. doi: 10.3389/fcimb.2022.953785. eCollection 2022. PubMed

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