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PB-TRE-OKSM
(Plasmid #204498)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204498 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 204498-D50 50 μg of DNA in Tris buffer $495
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DNA 204498-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    OCT4
  • Species
    H. sapiens (human)
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OCT4Borf1, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)

Gene/Insert 2

  • Gene/Insert name
    KLF4
  • Species
    H. sapiens (human)
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)

Gene/Insert 3

  • Gene/Insert name
    SOX2
  • Species
    H. sapiens (human)
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    OKSM fragment was obtained from pMaster3 (Addgene plasmid #58526) (Wu, Wu, Zhang, & Capecchi, PNAS, 2014), with the modification of Klf4 according to a previous report (Kim et al., Stem Cell Reports, 2015)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-TRE-OKSM was a gift from Wataru Fujii (Addgene plasmid # 204498 ; http://n2t.net/addgene:204498 ; RRID:Addgene_204498)

  • For your References section:

    Establishment of African pygmy mouse induced pluripotent stem cells using defined doxycycline inducible transcription factors. Matsuya S, Fujino K, Imai H, Kusakabe KT, Fujii W, Kano K. Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.1038/s41598-024-53687-9. 10.1038/s41598-024-53687-9 PubMed 38331995
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