PB-TRE-GNL
(Plasmid
#204499)
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PurposeExpresses Glis1, Nanog and lin28a by tet-on promoter in mammalian cells
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Depositing Lab
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Depositing OrganizationThe University of Tokyo
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204499 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 204499-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 204499-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC19
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGlis1
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SpeciesM. musculus (mouse)
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Entrez GeneGlis1 (a.k.a. Gli5, Gli6, GliH1)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The NL fragment was obtained from pMaster3 (Addgene plasmid #58526) (Wu et al., 2014)
DNA (Catalog # 204499-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 204499-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-TRE-GNL was a gift from Wataru Fujii (Addgene plasmid # 204499 ; http://n2t.net/addgene:204499 ; RRID:Addgene_204499) -
For your References section:
Establishment of African pygmy mouse induced pluripotent stem cells using defined doxycycline inducible transcription factors. Matsuya S, Fujino K, Imai H, Kusakabe KT, Fujii W, Kano K. Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.1038/s41598-024-53687-9. 10.1038/s41598-024-53687-9 PubMed 38331995
