pCAG-T3-HypaCAS9-pA
(Plasmid #131467)

• Purpose: Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
• Depositing Lab: Wataru Fujii
• Publication: Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.1038/s42003-019-0627-8. eCollection 2019.

Backbone

• Vector backbone: pCAGGS
• Total vector size (bp): 9293
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Codon optimized HypaCas9
• Species: Synthetic
• Insert Size (bp): 4218
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)
  • polyA (C terminal on insert)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid vector was constructed by mutation PCR of pCAG-T3-hCAS9-pA plasmid vector (Addgene plasmid 48625) according to the amino acid sequences of HypaCas9 shown in previous study (Chen JS., Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Nature, 2017).