pCAG-T3-st1CAS9-pA
(Plasmid #131542)

• Purpose: Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
• Depositing Lab: Wataru Fujii
• Publication: Fujii et al Biochem Biophys Res Commun. 2016 Jun 16. pii: S0006-291X(16)30988-3. doi: 10.1016/j.bbrc.2016.06.070.

Backbone

• Vector backbone: pCAG-T3-hCAS9-pA
• Total vector size (bp): 8538
• Modifications to backbone: The human codon optimized St1-Cas9 ORF, which was amplified by PCR using the plasmid (Addgene #48669), was inserted into the NotI to ClaI site of pCAG-T3-hCAS9-pA plasmid (Addgene #48625).
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Codon optimized st1Cas9
• Species: Synthetic
• Insert Size (bp): 3455
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)
  • polyA (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The human codon-optimized st1Cas9 insert in this vector was amplified from plasmid M-ST1cas (George Church; Addgene #48669).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry