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PurposeThe plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 173897 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepKW2
- Total vector size (bp) 5860
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameSepRS(2)
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SpeciesMethanococcus maripaludis
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Insert Size (bp)1617
Gene/Insert 2
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Gene/Insert namepSertRNA
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Insert Size (bp)75
Gene/Insert 3
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Gene/Insert nameEF-Sep
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Insert Size (bp)1299
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SepRS(2)/pSertRNA(B4)/EF-Sep was a gift from Jason W Chin (Addgene plasmid # 173897 ; http://n2t.net/addgene:173897 ; RRID:Addgene_173897) -
For your References section:
Efficient genetic encoding of phosphoserine and its nonhydrolyzable analog. Rogerson DT, Sachdeva A, Wang K, Haq T, Kazlauskaite A, Hancock SM, Huguenin-Dezot N, Muqit MM, Fry AM, Bayliss R, Chin JW. Nat Chem Biol. 2015 Jul;11(7):496-503. doi: 10.1038/nchembio.1823. Epub 2015 Jun 1. 10.1038/nchembio.1823 PubMed 26030730
