We narrowed to 3,718 results for: yfp

1. G protein alpha-q-CFP

   Plasmid

   #66081

   Purpose

   This G protein alpha-q construct contains internal insertions of CFP and the EE epitope

   Depositor

   Catherine Berlot

   Article

   Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.

   Insert

   G protein alpha-q-EE-CFP (Gnaq Mouse)
   

   Tags

   CFP was inserted internally into the alpha-q sequ…

   Expression

   Mammalian

   Mutation

   BamHI and SacI sites in alpha-q were removed with…

   Promoter

   CMV

   Available Since

   July 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYEE

   Plasmid

   #159750

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CFP(159-238)-gamma-2 in pcDNAI/Amp

   Plasmid

   #55777

   Purpose

   A C-terminal CFP fragment was fused to Ggamma-2. When co-expressed with an N-terminal mCerulean, CFP, or YFP fragment fused to a Gbeta subunit with which it interacts a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   CFP(159-238)-gamma-2 (GNG2 Human, Aequorea victoria)
   

   Tags

   CFP(159-238) was fused to the amino terminus of G…

   Expression

   Mammalian

   Mutation

   CFP(159-238) includes a substitution of His for A…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CFP(159-238)-beta-5 in pcDNAI/Amp

   Plasmid

   #55763

   Purpose

   A carboxyl-terminal CFP fragment was fused to Gbeta-5. When co-expressed with an amino terminal CFP or YFP fragment fused to a Ggamma subunit or RGS7, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   CFP(159-238)-beta-5 (GNB5 Human, Aequorea victoria)
   

   Tags

   CFP(159-238) was fused to the amino terminus of G…

   Expression

   Mammalian

   Mutation

   CFP(159-238) includes a substitution of His for A…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. S10A mutant of H3S10ph biosensor

   Plasmid

   #120810

   Purpose

   FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
   

   Expression

   Mammalian

   Mutation

   Serine 10 is mutated to Alanine, and Threonine 3,…

   Promoter

   CMV

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Desmoplakin I affinity mutant (F40-based tension sensor)

   Plasmid

   #119190

   Purpose

   The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AURKA-Aquamarine

   Plasmid

   #228564

   Purpose

   Encodes a donor-only, Aquamarine-tagged version of the AURKA biosensor under the control of a CMV promoter

   Depositor

   Giulia Bertolin

   Article

   Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

   Insert

   AURKA (AURKA, AURKA Human)
   

   Tags

   Aquamarine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-CMV-MLH1dn-IRES-puroR

   Plasmid

   #207354

   Purpose

   Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   MutL homolog 1 (MLH1 Human)
   

   Use

   Lentiviral

   Mutation

   Δ754-756 dominant negative mutant

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CFP(159-238)-beta-1 in pcDNAI/Amp

   Plasmid

   #55592

   Purpose

   A carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

   Insert

   CFP (159-238)-Beta 1 (GNB1 Human, Aequorea victoria)
   

   Tags

   CFP(159-238) was fused to the amino terminus of G…

   Expression

   Mammalian

   Mutation

   CFP(159-238) includes a substitution of His for A…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

    Plasmid

    #156415

    Purpose

    kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    mTagBFP2

    Mutation

    mutant KRAS4A-G12D

    Promoter

    kdrl

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

    Plasmid

    #156414

    Purpose

    kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4B-G12V (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    EGFP

    Mutation

    mutant KRAS4B-G12V

    Promoter

    kdrl

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJEL061

    Plasmid

    #24016

    Depositor

    George Cross

    Type

    Empty backbone

    Use

    Trypanosome expression

    Tags

    EYFP

    Available Since

    Feb. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KRD22_HBA1(HBA1reg-HBB-longHAs)

    Plasmid

    #232403

    Purpose

    AAV production plasmid for HBA1 long HAs vector from Figs. 3-6 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank full HBA1-2A-YFP cassette. HAs are ~900bp

    Depositor

    Kyle Cromer, Matthew Porteus

    Article

    Cromer et al Nat Med. 2021 Apr;27(4):677-687. doi: 10

    Inserts

    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Human Hemoglobin Subunit Beta (HBB Human)
    

    Expression

    Mammalian

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVenus-N1 (804)

    Plasmid

    #61854

    Purpose

    empty backbone for tagging proteins at the C-terminus with Venus

    Depositor

    Jonathon Pines

    Type

    Empty backbone

    Tags

    Venus

    Expression

    Mammalian

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL2_213

    Plasmid

    #210697

    Purpose

    Even ORI-marker screen destination vector; contains sfYFP dropout for barcoded-ORI insertion

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL2_212

    Plasmid

    #210696

    Purpose

    Even ORI-marker screen destination vector; contains sfYFP dropout for barcoded-ORI insertion

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL2_211

    Plasmid

    #210695

    Purpose

    Even ORI-marker screen destination vector; contains sfYFP dropout for barcoded-ORI insertion

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIC241

    Plasmid

    #69739

    Purpose

    Retroviral expression of Sgo-1

    Depositor

    Iain Cheeseman

    Insert

    Sgo-1 (SGO1 Human)
    

    Use

    Retroviral

    Tags

    eYFP-TEV-S

    Expression

    Mammalian

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pIC195

    Plasmid

    #69730

    Purpose

    Retroviral expression of Polo-Like Kinase 1 (Plk1)

    Depositor

    Iain Cheeseman

    Insert

    Polo-Like Kinase 1 (Plk1) (PLK1 Human)
    

    Use

    Retroviral

    Tags

    eYFP-TEV-S

    Expression

    Mammalian

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIC234

    Plasmid

    #69733

    Purpose

    Retroviral expression of Nup-37

    Depositor

    Iain Cheeseman

    Insert

    Nup-37 (NUP37 Human)
    

    Use

    Retroviral

    Tags

    eYFP-TEV-S

    Expression

    Mammalian

    Mutation

    E121K compared to GenBank reference sequence NP_0…

    Available Since

    Dec. 3, 2015

    Availability

    Academic Institutions and Nonprofits only