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AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE

Plasmid

#129704

Purpose

Express HA-KASH for nuclei labeling and purification

Depositor

Yi Zhang

Article

Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

Insert

HA-VHH-KASH

Use

AAV and Cre/Lox

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-NEUROD1-T2A-mCherry

Plasmid

#178584

Purpose

Cre-dependent expression of NEUROD1 and mCherry under the constitutive promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

NEUROD1 (NEUROD1 Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-EGFP barcode-10-SV40 polyA

Plasmid

#190874

Purpose

For barcoded retrograde labeling

Depositor

Yuejun Chen

Article

Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

Insert

EGFP-barcode10

Use

AAV

Tags

Expression

Mutation

WT

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

Plasmid

#112684

Purpose

An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

iRFP713

Use

AAV

Tags

2A-Flpo and Nuclear localization signal

Expression

Mutation

Promoter

CMV-IE

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP

Plasmid

#47905

Purpose

An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

hChR2(H134R)

Use

AAV

Tags

iRFP

Expression

Mammalian

Mutation

H134R

Promoter

CaMKIIa

Available since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-SID4X-pU6-sgRNA

Plasmid

#158989

Purpose

Vector F encodes pAAV-pMecp2-dSaCas9-SID4X-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-SID4X

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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AAV Cag Flex H2B Gcamp6s reverse

Plasmid

#74157

Purpose

Calcium Sensor

Depositor

Loren Looger

Insert

Gcamp6s

Use

AAV

Tags

H2B

Expression

Mutation

Promoter

SpeI

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-LMO3 (sbGLuc-VChR1-EYFP)

Plasmid

#114104

Purpose

fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

sbGLuc-VChR1-EYFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA

Plasmid

#109318

Purpose

Plasmid for liver-specific expression of EmGFP with a gRNA against SaCas9

Depositor

William Lagor

Article

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Insert

SaCas9 RuvC gRNA

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA

Plasmid

#37089

Purpose

Expresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

ChETA-TdTomato

Use

AAV and Cre/Lox; Cre-off

Tags

TdTomato

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only
AAV-CAG-FLEX-LifeAct-jGCaMP8f-WPRE

Plasmid

#186047

Purpose

AAV-mediated expression of LifeAct-jGCaMP8f under the CAG promoter, Cre-dependent expression

Depositor

Loren Looger

Insert

LifeAct-jGCaMP8f

Use

AAV

Tags

LifeAct-6xHis

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLEX-LifeAct-jGCaMP8m-WPRE

Plasmid

#186048

Purpose

AAV-mediated expression of LifeAct-jGCaMP8m under the CAG promoter, Cre-dependent expression

Depositor

Loren Looger

Insert

LifeAct-jGCaMP8m

Use

AAV

Tags

LifeAct-6xHis

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-tmeGFP-IRES-nls-mRuby2

Plasmid

#63060

Purpose

Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

nls-mRuby2

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP

Plasmid

#55642

Purpose

sCre-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

hChR2(H134R)-EYFP

Use

AAV; Scre-dependent

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)

Plasmid

#114101

Purpose

fusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

Insert

Gaussia luciferase, M23; Volvox Channelrhodopsin 1; EYFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNA

Plasmid

#194727

Purpose

CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA array targeting hAAVS1.

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1(D908A) (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC710 - pAAV EF1a DIO Mem-AcGFP

Plasmid

#75081

Purpose

An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

Insert

Mem-AcGFP

Use

AAV and Cre/Lox

Tags

Palmitylation site

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)

Plasmid

#155194

Purpose

Expresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Truncated sequence of THBS1 (expresses Amino Acid…

Promoter

CMV

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Linker-hSyn::mCherry.3xFLAG-WPRE

Plasmid

#120391

Purpose

pAAV plasmid expressing mCherry.3XFLAG under the hSyn promoter with a Linker containing BstBI, BspQI and SacI sites upstream of the hSyn promoter

Depositor

Florian Freudenberg

Article

Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

Type

Empty backbone

Use

AAV

Tags

mCherry.3XFLAG

Expression

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-Archon1-KGC-GFP-ER2

Plasmid

#115890

Purpose

AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-GFP-ER2

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Mutation

Promoter

EF1a（1.1kb short version)

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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