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Plasmid#86634PurposeExpresses FRB-tagged human Akt1(D274A) kinase-inactive mutantDepositorInsertFRB-Akt1(D274A) (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM1157
Plasmid#112534PurposepZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanRDepositorInsertsHrtR
luxCDABE
UseTagsExpressionBacterialMutationPromoterPL(HrtO) and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHlip-GFP-HiBit
Plasmid#171016PurposeFor bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHlip-muGFP-HiBiT
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIS1-wt RhoA 3'UTR
Plasmid#26089DepositorInsertwt RhoA 3'UTR (RHOA Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-sbGLuc-B7-EYFP
Plasmid#114110Purposefusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogeneticsDepositorInsertsGaussia luciferase, slow burn
B7 hinge region
Enhanced Yellow Fluorescent Protein
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-PH1(K14A)
Plasmid#86600PurposeExpresses mCherry-tagged PH(K14A) domain of human Akt1DepositorInsertK14A mutant of Akt1 PH domain (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
-111 hTF m3
Plasmid#15450DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseTagsExpressionMammalianMutationAll 3 EGR-1 sites mutatedPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(K14A)
Plasmid#86562PurposeExpresses human full-length Akt1(K14A) mutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationK14A mutation in the PH domainPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMEH60 Lamp2b MS2 HA
Plasmid#168209PurposeMammalian expression of lysosome-associated membrane protein 2b fused to the MS2 dimer and C-terminal HA tagDepositorInsertLamp2b (LAMP2 Human)
UseTagsHA and MS2 dimerExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPTXcGMPRELUC
Plasmid#68503Purposecyclic nucleotide reporter for bacteria or plant cellsDepositorInsertOPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
UseTagsluciferaseExpressionBacterial and PlantMutation3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…PromoterOPTX promoter with cGMPREAvailable sinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
-111 hTF m4
Plasmid#15451DepositorInsertTissue factor promoter (F3 Human)
UseLuciferaseTagsExpressionMammalianMutationAll 3 Sp1 and EGR-1 sites mutatedPromoterAvailable sinceJuly 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 4xSBR2 MutSBE
Plasmid#15707DepositorInsertp15 4xSBR2 (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationMutant SBE (Smad binding element)PromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGL2 p15 4xSBR1 MutSBE
Plasmid#15718DepositorInsertp15 4xSBR1 (CDKN2B Human)
UseLuciferaseTagsExpressionMammalianMutationMutant SBE (Smad binding element)PromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
psiCheck-ApoE3'UTR
Plasmid#41846DepositorInsertApoE 3'UTR (APOE Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(D323A/D325A)
Plasmid#86592PurposeExpresses human full-length Akt1(D323A/D325A) mutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationD323A/D325A transforming mutationsPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG179 BC072452 3'UTR mut
Plasmid#12037DepositorInsertN12 3'UTR mut (FAM107B Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOPTXGARE
Plasmid#68542Purposecyclic nucleotide reporter for bacteria or plant cellsDepositorInsertOPTX promoter with GARE (AT1G33440 Mustard Weed)
UseTagsluciferaseExpressionBacterial and PlantMutation5x GARE inserted 190 bp 5' pf ATG (GARE = ta…PromoterOPTX promoter with GAREAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB785
Plasmid#60164PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB787
Plasmid#60152PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB789
Plasmid#60166PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only