We narrowed to 5,231 results for: golden gate

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pEV785

Plasmid

#246451

Purpose

The plasmid comprises an indicible transcriptional unit encoding Gal4-VPR

Depositor

Ron Weiss

Publication

Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038

Insert

Gal4-VPR

Use

Synthetic Biology

Mutation

Mutated Kozak sequence

Promoter

miniYB

Available since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1729

Plasmid

#246063

Purpose

Bacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a ori

Depositor

Jennifer Doudna

Publication

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Panoptes locus

Expression

Bacterial

Promoter

pTet promoter

Available since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1540

Plasmid

#246061

Purpose

Bacterial expression of 2TMbeta protein under the pJex promoter

Depositor

Jennifer Doudna

Publication

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

2TMbeta

Expression

Bacterial

Promoter

pJEX promoter

Available since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pBA1326

Plasmid

#246058

Purpose

Bacterial expression of RFP under control of the pJEX promoter

Depositor

Jennifer Doudna

Publication

Doherty et al Nature. 2025 Nov;647(8091):997-1004. doi

Insert

Red Fluorescent Protein

Expression

Bacterial

Promoter

pJEX promoter

Available since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1272 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-2GG+rpoA-IP3

Plasmid

#235120

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses IP3.

Depositor

Oscar Ramos

Publication

Guyot et al Comput Struct Biotechnol J. 2026 May 25;

Type

Empty backbone

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

rpoA

Expression

Bacterial

Available since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411b

Plasmid

#246583

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Publication

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, AtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 )

Expression

Bacterial

Available since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW334e2b

Plasmid

#246587

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Publication

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, AtRbcX2, AtBSD2W108A/L109E, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN60B, CPN60A, CPN20, RbcX2 Mustard Weed)

Expression

Bacterial

Mutation

AtBSD2W108A/L109E

Available since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW334e4

Plasmid

#246589

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Publication

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN20, RbcX2, CPN60B, CPN60A Mustard Weed)

Expression

Bacterial

Available since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW334e2

Plasmid

#246586

Purpose

sequential knock out of A. thaliana Rubisco assembly factors

Depositor

Tina Wang

Publication

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, AtRbcX2, AtCpn60alpha, AtCpn60beta, AtCpn20 (AT3G04550, CPN60B, CPN60A, CPN20, RbcX2 Mustard Weed)

Expression

Bacterial

Available since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411c

Plasmid

#246584

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Publication

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, NtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 Mustard Weed)

Expression

Bacterial

Available since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411a

Plasmid

#246582

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Publication

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1, AtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN20, CPN60A, AT3G04550 Mustard Weed)

Expression

Bacterial

Available since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTB105

Plasmid

#236778

Purpose

Backbone for cloning sgRNAs to be used with CRISPR Cas9 cytosine base editor. Expressed from the 18S rRNA SSU locus in Leishmania. Contains puromycin selection marker.

Depositor

Tom Beneke

Publication

Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7

Type

Empty backbone

Use

CRISPR

Available since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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cwby0015

Plasmid

#232224

Purpose

GGA cloning his-mhfYFP-AGGA-promoter-ccdb-TTCC

Depositor

David Baker

Publication

Qian et al bioRxiv 2025.08.05.668824; doi: https://

Insert

ccdb

Expression

Bacterial

Promoter

T7

Available since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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cwby0013

Plasmid

#232222

Purpose

GGA cloning his-muGFP-AGGA-promoter-ccdb-TTCC

Depositor

David Baker

Publication

Qian et al bioRxiv 2025.08.05.668824; doi: https://

Insert

ccdb

Expression

Bacterial

Promoter

T7

Available since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L4866 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF1a in MGEV

Plasmid

#244190

Purpose

Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF1a

Depositor

Joshua Leonard

Publication

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF1 synTF

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Mutation

NTEVp_75S, CTEVp_190K

Promoter

hEF1a, CMV

Available since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L4864 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF6a in MGEV

Plasmid

#244189

Purpose

Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF6a

Depositor

Joshua Leonard

Publication

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF6 synTF

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Mutation

NTEVp_75S, CTEVp_190K

Promoter

hEF1a, CMV

Available since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L3928 Filler DNA

Plasmid

#244193

Purpose

Empty vector; modified pcDNA with CMV promoter and bGHpolyA removed to reduce burden

Depositor

Joshua Leonard

Publication

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L4869 hVEGFA-121 in pcDNA backbone

Plasmid

#244180

Purpose

Constitutive expression of human VEGFA-121 isoform

Depositor

Joshua Leonard

Publication

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

VEGFA-121 (VEGFA Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L4278 hIgKLSS_hTGFb1 in pcDNA backbone

Plasmid

#244184

Purpose

Constitutive expression of human TGF-beta1 (with the human IgK light chain secretion sequence)

Depositor

Joshua Leonard

Publication

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

TGF-beta1 with IgK light chain secretion sequence (TGFB1 Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L4275 hIgKLSS_hTNF in pcDNA backbone

Plasmid

#244182

Purpose

Constitutive expression of human mature TNF (with the human IgK light chain secretion sequence)

Depositor

Joshua Leonard

Publication

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

TNF with IgK light chain secretion sequence (TNF Human, Synthetic)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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