We narrowed to 6,070 results for: nls

1. pET-21a-PE2-His

   Plasmid

   #201973

   Purpose

   Express His-tagged PE2 in bacteria

   Depositor

   Scot Wolfe

   Article

   Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

   Insert

   PE2-HIS
   

   Use

   Tags

   His and c-myc NLS

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4396

   Plasmid

   #74041

   Purpose

   Expresses human codon-optimized AsCpf1 and As crRNA.

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   As crRNA
   AsCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSN067

   Plasmid

   #101748

   Purpose

   Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Inserts

   Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
   KanMX marker expression cassette.
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   Heterologous TEF1 promoter from A. gossypii. and …

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-nef-CIAO2-Flp

   Plasmid

   #149296

   Purpose

   Cre-dependent expression of Flp recombinase.

   Depositor

   Edward Callaway

   Article

   Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

   Insert

   Flp recombinase (flp Budding Yeast)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Derived from the mouse codon-optomized FlpO. The…

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRDA_763

   Plasmid

   #201156

   Purpose

   EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' NLS for CRISPRa

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   5x-ALFA tag-dEnAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D908A

   Promoter

   EFS

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LLP777_SnoopCatcher-p65HSF1-CO

   Plasmid

   #211770

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SnoopCatcher-p65HSF1
   

   Use

   Tags

   2xOLLAS

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LLP775_αGCN4-p65HSF1-CO

   Plasmid

   #211768

   Purpose

   SunTag counterpart binding domain, aGCN4, fused to transcriptional activator p65HSF1, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   aGCN4-p65HSF1
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect p65HSF…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nef-CIAO-Flp

   Plasmid

   #149294

   Purpose

   Cre-dependent expression of Flp recombinase.

   Depositor

   Edward Callaway

   Article

   Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

   Insert

   Flp recombinase (flp Budding Yeast)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Derived from the mouse codon-optomized FlpO. The…

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-BE4-PpAPOBEC1 R33A

   Plasmid

   #138345

   Purpose

   Mammalian expression plasmid for BE4-PpABOBEC1 R33A

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-PpAPOBEC1 R33A
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Expression

   Mutation

   Change of Arg 33 to Ala.

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiAsCpf1-3xMYC-blast

    Plasmid

    #127750

    Purpose

    Lentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal

    Depositor

    Giulio Draetta

    Article

    Liu et al Nat Commun. 2019 Jul 17;10(1):3144. doi:

    Insert

    AsCpf1
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104911

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. LLP778_Traptavidin-p65HSF1-CO

    Plasmid

    #211771

    Purpose

    AviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavidin-p65HSF1
    

    Use

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP776_SpyCatcher-p65HSF1-CO

    Plasmid

    #211769

    Purpose

    SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    SpyCatcher-p65HSF1
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect p65HSF…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ220 (AsCpf1)

    Plasmid

    #86208

    Purpose

    AsCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    AsCpf1
    

    Use

    Gateway compatible cpf1 vectors

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-LbCpf1-Blast

    Plasmid

    #84751

    Purpose

    Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    LbCpf1
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-3xHA (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSN068

    Plasmid

    #101749

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGAPDH-Cre-HYG

    Plasmid

    #231510

    Purpose

    Expresses Cre recombinase in Acanthamoeba

    Depositor

    Yeonchul Hong

    Insert

    Cre recombinase
    

    Use

    Unspecified; Acanthamoeba castellanii neff strain

    Tags

    EGFP and SV40 NLS

    Expression

    Mutation

    Promoter

    Acanthamoeba GAPDH

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pM125: pAAV-EFS-CasRx-VEGFA array presgRNA

    Plasmid

    #166873

    Purpose

    AAV vector for expressing CasRx and human VEGFA targeting presgRNA array for RNA-editing

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Inserts

    U6-VEGFA array presgRNA
    RfxCas13d
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY011 (pcDNA3.1-hLb2Cpf1)

    Plasmid

    #69983

    Purpose

    Expresses humanized Lb2Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb2Cpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. (364) SB KRAB-SadCas9

    Plasmid

    #163022

    Purpose

    Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    Transposon

    Tags

    KRAB and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TET-On

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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