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Showing: 4161 - 4180 of 9426 results

  1. pDONR223 SARS-CoV-2 NSP7_nostop

    Plasmid
    #149310
    Purpose
    Gateway-compatible Entry vector, with insert of NSP7 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP7 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149310-plasmid-map-sequence-id-296439

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  2. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

    Plasmid
    #113692
    Purpose
    U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 (NEWENTRY )
    SaCas9 gRNA Cassette (NEWENTRY )
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113692-plasmid-map-sequence-id-229557

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  3. Epac-S-H94

    Plasmid
    #170341
    Purpose
    GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    GFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    170341-plasmid-map-sequence-id-335921

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  4. pDONR223 SARS-CoV-2 NSP4_nostop

    Plasmid
    #149307
    Purpose
    Gateway-compatible Entry vector, with insert of NSP4 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149307-plasmid-map-sequence-id-296435

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  5. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

    Plasmid
    #105805
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105805-plasmid-map-sequence-id-207906

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  6. pDONR223 SARS-CoV-2 NSP8_nostop

    Plasmid
    #149311
    Purpose
    Gateway-compatible Entry vector, with insert of NSP8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149311-plasmid-map-sequence-id-296441

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  7. Epac-S-H150

    Plasmid
    #170345
    Purpose
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    170345-plasmid-map-sequence-id-344646

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  8. pDONR223 SARS-CoV-2 S 24nt-del

    Plasmid
    #153952
    Purpose
    Gateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    June 23, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153952-plasmid-map-sequence-id-300778

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  9. pDONR223 SARS-CoV-2 S 24nt-del_nostop

    Plasmid
    #153953
    Purpose
    Gateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    June 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153953-plasmid-map-sequence-id-307591

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  10. pKK-BI16-ORF1-3C-FLAG_ORF2-TEV-mClover3

    Plasmid
    #105803
    Purpose
    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C protease; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-FLAG; ORF2: TEV-mClover3
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105803-plasmid-map-sequence-id-203399

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  11. pBabe-kappa-HA-dL5-myc-ADRB2

    Plasmid
    #101253
    Purpose
    Expresses HA-dL5(E52D)-cMyc N-terminal fusion of beta-2 adrenergic receptor in mammalian cells, MMLV retroviral plasmid (MBIC5, dL5**, FAP, ADRB2)
    Depositor
    Marcel Bruchez
    Article
    Perkins et al Biochemistry. 2018 Feb 6;57(5):861-871.
    Insert
    kappa-HA-dL5-myc-ADRB2 (ADRB2 Budding Yeast, Human)
    Use
    Retroviral
    Tags
    FAP-dL5 and HA epitope
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV LTR
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    101253-plasmid-map-sequence-id-235286

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  12. pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop

    Plasmid
    #153956
    Purpose
    Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    June 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153956-plasmid-map-sequence-id-299762

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  13. pDONR223 SARS-CoV-2 ORF7B C-trunc

    Plasmid
    #153957
    Purpose
    Gateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    June 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153957-plasmid-map-sequence-id-299766

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  14. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber

    Plasmid
    #105806
    Purpose
    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: 3C-Cerulean; ORF2: TEV-Amber
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    105806-plasmid-map-sequence-id-207907

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  16. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-seq2

    Plasmid
    #240865
    Purpose
    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes
    Depositor
    Mark Verheijen
    Article
    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23
    Insert
    U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
    Use
    AAV and CRISPR
    Tags
    SaCas9 + 3X HA-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    GfaABC1D
    Available since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240865-plasmid-map-sequence-id-482051

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  17. pAAV- GfaABC1D::Cas9-HA-sgRNA-Ezr-Seq1

    Plasmid
    #240094
    Purpose
    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of sgRNA targeting exon-1 of mouse Ezrin; for knocking-out ezrin in murine astrocytes
    Depositor
    Mark Verheijen
    Article
    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23
    Insert
    U6 driven sgRNA Targeting exon 1 of EZR (Ezr Mouse)
    Use
    AAV and CRISPR
    Tags
    SaCas9 + 3X HA-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    GfaABC1D
    Available since
    Aug. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240094-plasmid-map-sequence-id-482052

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  18. pcDNA3.1(+)_Nav1.6(K1425TAG, Y371C)-HA

    Plasmid
    #239766
    Purpose
    Expresses TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1425 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060
    Insert
    Scn8a (Scn8a Mouse)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    K1425TAG, Y371C
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239766-plasmid-map-sequence-id-477737

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  19. pcDNA3.1(+)_Nav1.6(K1546TAG, Y371C)-HA

    Plasmid
    #239767
    Purpose
    Expresses TTX-resistant (Y371C) variant of mouse Nav1.6 with a TAG codon at position 1546 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060
    Insert
    Scn8a (Scn8a Mouse)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    K1546TAG, Y371C
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239767-plasmid-map-sequence-id-477107

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Showing: 4161 - 4180 of 9426 results