We narrowed to 10,132 results for: yeast

1. pDONR221-Ckb1

   Plasmid

   #99981

   Purpose

   Shuttle yeast Ckb1 into Gateway destination vector

   Depositor

   Susan Baserga

   Article

   Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

   Insert

   Ckb1 (CKB1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-Ckb2

   Plasmid

   #99982

   Purpose

   Shuttle yeast Ckb2 into Gateway destination vector

   Depositor

   Susan Baserga

   Article

   Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

   Insert

   Ckb2 (CKB2 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221-Utp11

   Plasmid

   #99984

   Purpose

   Shuttle yeast Utp11 into Gateway destination vector

   Depositor

   Susan Baserga

   Article

   Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

   Insert

   Utp11 (UTP11 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-Pfa1

   Plasmid

   #99999

   Purpose

   Shuttle yeast Pfa1 into Gateway destination vector

   Depositor

   Susan Baserga

   Article

   Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

   Insert

   Pfa1 (SQS1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-Cka1

   Plasmid

   #99979

   Purpose

   Shuttle yeast Cka1 into Gateway destination vector

   Depositor

   Susan Baserga

   Article

   Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

   Insert

   Cka1 (CKA1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMES140

   Plasmid

   #71849

   Purpose

   Complementation of NME1 gene disruption or mutation in yeast

   Depositor

   David Clayton, Mark Schmitt

   Article

   Schmitt et al Mol Cell Biol. 1993 Dec;13(12):7935-41.

   Insert

   NME1 (NME1 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   NME1

   Available Since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDS3-X4

   Plasmid

   #70140

   Purpose

   Yeast chr. integration vector with DS3 selection marker

   Depositor

   Morten Sommer

   Article

   Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

   Insert

   DS3
   

   Expression

   Yeast

   Promoter

   AgTEF1

   Available Since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZHY566

   Plasmid

   #50608

   Purpose

   TALEN expression vector for yeast, N152,C18 architecture

   Depositor

   Daniel Voytas

   Article

   Zhang et al Plant Physiol. 2012 Nov 2.

   Type

   Empty backbone

   Use

   TALEN

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZHY565

   Plasmid

   #50607

   Purpose

   TALEN expression vector for yeast, N152,C18 architecture

   Depositor

   Daniel Voytas

   Article

   Zhang et al Plant Physiol. 2012 Nov 2.

   Type

   Empty backbone

   Use

   TALEN

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   Jan. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSF-KpDcr1deltaC(GiVL-1)

    Plasmid

    #32032

    Depositor

    David Bartel

    Article

    Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

    Insert

    DCR1(15-355)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    H123-N143 replaced by P642-Y645 of GiDicer

    Promoter

    T7

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSF-KpDcr1(1-384)

    Plasmid

    #32031

    Depositor

    David Bartel

    Article

    Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

    Insert

    DCR1(1-384)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSF-KpDcr1deltaC(GiVL-2)

    Plasmid

    #32034

    Depositor

    David Bartel

    Article

    Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

    Insert

    DCR1(15-355)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    N195-M215 replaced by P697-D714 of GiDicer

    Promoter

    T7

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSF-KpDcr1deltaC(E147Q/D151N)

    Plasmid

    #32024

    Depositor

    David Bartel

    Article

    Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

    Insert

    DCR1(15-355)
    

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    E147Q/D151N

    Promoter

    T7

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAS

    Plasmid

    #60847

    Purpose

    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

    Depositor

    Jamie Cate

    Article

    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

    Inserts

    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    NLS/His8 and TTT 3' extension prior to sgRNA

    Expression

    Bacterial and Yeast

    Mutation

    L4 is UUCG tetraloop and guide targets LYP1 (CATA…

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBridge-tyrosinase.σ2

    Plasmid

    #197415

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-2 σ2
    

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    encodes R42G substitution, contains silent substi…

    Promoter

    ADH1 and MET25

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG414GPD-dCas9-VPR

    Plasmid

    #63801

    Purpose

    pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Yeast

    Promoter

    GPD

    Available Since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIFU002

    Plasmid

    #174299

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HA and cMyc

    Expression

    Yeast

    Mutation

    N343Q, N501Y

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIFU001

    Plasmid

    #174298

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    HA and cMyc

    Expression

    Yeast

    Mutation

    N343Q, E484K

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJC20cdc8.A18T

    Plasmid

    #99360

    Purpose

    Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    Alanine 18 to Threonine

    Promoter

    T7

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJC20cdc8.E31K

    Plasmid

    #99361

    Purpose

    Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    Glutamic acid 31 to Lysine

    Promoter

    T7

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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