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pCMV2 HuOpt Bxb1

Plasmid

#242869

Purpose

Mammalian expression of Bxb1 Integrase

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMOD_C2911

Plasmid

#161764

Purpose

MODULE C plasmid compatible with the Voytas Plant Genome Engineering Tool kit with an Agrobacterium promoter rolD expressing the TREX2 exonuclease.

Depositor

Shaun Curtin

Publication

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

rolD:TREX2

Expression

Bacterial

Promoter

rolD

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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p09008_AF1-4+GFP5-7

Plasmid

#173715

Purpose

Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

Depositor

Johannes Kabisch

Publication

Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

Inserts

hybrid GFP
mKate2

Use

E. coli cell free protein synthesis extracts

Expression

Bacterial

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c15

Plasmid

#242873

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (V122M, E434G)

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

V122M, E434G

Promoter

CMV

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c10

Plasmid

#242872

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L, E434G)

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

I87L, E434G

Promoter

CMV

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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SM471_EF1A-dEnAsCas12a-T2A-BlastR

Plasmid

#244223

Purpose

Mammalian expression of dEnAsCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-shControl-mCherry-CAAX

Plasmid

#227689

Purpose

Membrane tethered mCherry and scramble of shRNA targeting Atg7

Depositor

Cagla Eroglu

Publication

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

scrambled shRNA control

Expression

Mammalian

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1-Pls2-sfGFP

Plasmid

#205749

Purpose

Expresses sfGFP under IPTG inducible promoter Pls2 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/low strength.

Depositor

Jerome Bonnet

Publication

Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

Type

Empty backbone

Use

Shuttle vector gram+ gram-

Expression

Bacterial

Promoter

Pls2 (Phyperspank mutant, IPTG inducible)

Available since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pZG162

Plasmid

#202581

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-hptII

Depositor

Guoyong Xu

Publication

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pZG164

Plasmid

#202583

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-hptII

Depositor

Guoyong Xu

Publication

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pZG163

Plasmid

#202582

Purpose

RNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-hptII

Depositor

Guoyong Xu

Publication

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pZG165

Plasmid

#202584

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-bar

Depositor

Guoyong Xu

Publication

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pZG161

Plasmid

#202580

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-bar

Depositor

Guoyong Xu

Publication

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pZG160

Plasmid

#202579

Purpose

RNA editing in plants. Plasmid contains extradiol-LIC cassette-dADARcd-35S-bar

Depositor

Guoyong Xu

Publication

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pZG159

Plasmid

#202578

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-35S-bar

Depositor

Guoyong Xu

Publication

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDGO186N-KS1

Plasmid

#174301

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

Depositor

Lee Lynd

Publication

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDGO186N-KS2

Plasmid

#174302

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

Depositor

Lee Lynd

Publication

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDGO186N-KS3

Plasmid

#174303

Purpose

Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

Depositor

Lee Lynd

Publication

Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

Insert

spacer expression cassette

Expression

Bacterial

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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SM274_EF1A-dEnAsCas12a-KRAB-T2A-EGFP

Plasmid

#244212

Purpose

Mammalian expression of dEnAsCas12a-KRAB with EGFP selectable marker

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM275_EF1A-dEnAsCas12a-KRAB-T2A-BlastR

Plasmid

#244213

Purpose

Mammalian expression of dEnAsCas12a-KRAB with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dEnAsCas12a-KRAB

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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