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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,132 results for: yeast

Showing: 5181 - 5200 of 10132 results

  1. pLH_Scr16

    Plasmid
    #100538
    Purpose
    CEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    Phytochrome B (PHYB Mustard Weed)
    Phytochrome interacting factor 3 (PIF3 Mustard Weed)
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Cdc1 NLS, CreC, CreN, and SV40 NLS
    Expression
    Yeast
    Mutation
    AA 1-621 of PhyB/ Mutation nucleotide 576 C-->A
    Promoter
    FBA1 and TDH3
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100538-plasmid-map-sequence-id-194462

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  2. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

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  3. pABD823

    Plasmid
    #67911
    Purpose
    Protein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiae
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    Cold temperature germinating 10 (CTG10)
    Tags
    GAL4 DNA binding domain (148 amino acids)
    Expression
    Yeast
    Promoter
    pADH1 (alcohol dehydrogenase 1 promoter)
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  4. pRS418

    Plasmid
    #11256
    Depositor
    Fred Cross
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Feb. 15, 2006
    Availability
    Academic Institutions and Nonprofits only
    11256-plasmid-map-sequence-id-6930

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  5. pRS428

    Plasmid
    #11257
    Depositor
    Fred Cross
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Feb. 15, 2006
    Availability
    Academic Institutions and Nonprofits only
    11257-plasmid-map-sequence-id-6931

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  6. pRS408

    Plasmid
    #11255
    Depositor
    Fred Cross
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Feb. 15, 2006
    Availability
    Academic Institutions and Nonprofits only
    11255-plasmid-map-sequence-id-6929

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  7. pRS420

    Plasmid
    #11259
    Depositor
    Fred Cross
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Feb. 15, 2006
    Availability
    Academic Institutions and Nonprofits only
    11259-plasmid-map-sequence-id-6933

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  8. pRS410

    Plasmid
    #11258
    Depositor
    Fred Cross
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Feb. 24, 2006
    Availability
    Academic Institutions and Nonprofits only
    11258-plasmid-map-sequence-id-6932

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  9. pDB4581

    Plasmid
    #171124
    Purpose
    A kanMX-marked pFA6a based plasmid, which has a 7-aa linker, a 16-aa XTEN16 linker, three tandam repeats of sAID. It serves as the template for 3_sAID degron tagging at the C-terminus.
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.
    Insert
    3_sAID
    Use
    Other
    Mutation
    non applicable
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171124-plasmid-map-sequence-id-336986

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  13. pRS159

    Plasmid
    #122378
    Purpose
    CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)
    Depositor
    Rebecca Shapiro
    Article
    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    122378-plasmid-map-sequence-id-241243

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  18. pCSN075

    Plasmid
    #166732
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available Since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166732-plasmid-map-sequence-id-332206

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  20. pAID6

    Plasmid
    #136989
    Purpose
    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4
    Depositor
    Huimin Zhao
    Article
    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:
    Inserts
    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    Expression
    Yeast
    Promoter
    ENO2, TDH3, TEF1, and TPI1
    Available Since
    May 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    136989-plasmid-map-sequence-id-269844

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Showing: 5181 - 5200 of 10132 results