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pAAV-Puro_siKD2.0

Plasmid

#220536

Purpose

AAVS1-targeting vector designed for the iPS2-seq platform to express a single-copy, tetracycline-inducible shRNA in human pluripotent stem cells

Depositor

Alessandro Bertero

Article

Balmas et al Mol Syst Biol. 2025 Dec 10. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-mRuby2-P2A-PuroR_pKG02755

Plasmid

#246371

Purpose

IVT template for making modRNA encoding mRuby2-P2A-PuroR

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology; In vitro transcription

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSYL017

Plasmid

#246593

Purpose

template plasmid for site-saturation mutagenesis library cloning

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1 variant 2g (AT3G04550 Mustard Weed)

Expression

Bacterial

Mutation

AtRaf1L121F/I127L/A195V/N351Y/R432G

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-mem-mScarlet-ORF-3

Plasmid

#241185

Purpose

Middle entry vector for mini-Golden subcloning platform; mScarlet

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mScarlet

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-mem-mScarlet-ORF-2

Plasmid

#241184

Purpose

Middle entry vector for mini-Golden subcloning platform; mScarlet

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mScarlet

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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linker-P2A-mem-mScarlet-ORF-1

Plasmid

#241183

Purpose

Middle entry vector for mini-Golden subcloning platform; mScarlet

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mScarlet

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-mNeongreen-ORF1

Plasmid

#241208

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-lifeact-mNeongreen-ORF-1

Plasmid

#241204

Purpose

Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

lifeact-mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-mNeongreen-ORF2

Plasmid

#241209

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-mNeongreen-ORF3

Plasmid

#241210

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_UCOE_TRE3VG_mCherry-Responder

Plasmid

#230053

Purpose

It presents the UCOE sequence and the TRE3VG inducible promoter allowing a more stable dox-dependent inducible activation of mCherry though the OPTi-OX platform across differentiation of hiPSCs

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

UCOE

Expression

Mammalian

Promoter

TRE3VG

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCLYBL-Neo_TRE3VG_mCherry_Responder

Plasmid

#230054

Purpose

It presents the TRE3VG inducible promoter allowing the dox-dependent inducible activation of mCherry through the OPTi-OX platform. The CLYBL GSH supports higher transgene expression

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

TRE3VG_mCherry

Expression

Mammalian

Promoter

TRE3VG

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-lifeact-mNeongreen-ORF-2

Plasmid

#241205

Purpose

Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

lifeact-mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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T2A-mCherry-P2A-NTRv2-pA-ORF-3

Plasmid

#241198

Purpose

Middle entry vector for mini-Golden subcloning platform; mCherry; NTR-v2

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry; NTR-v2

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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T2A-BFP-P2A-NTRv2-pA-ORF-2

Plasmid

#241200

Purpose

Middle entry vector for mini-Golden subcloning platform; BFP; NTR-v2

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP; NTR-v2

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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T2A-BFP-P2A-NTRv2-pA-ORF-3

Plasmid

#241201

Purpose

Middle entry vector for mini-Golden subcloning platform; BFP; NTR-v2

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

BFP; NTR-v2

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-mem-mScarlet-ORF-2

Plasmid

#241187

Purpose

Middle entry vector for mini-Golden subcloning platform; mScarlet

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mScarlet

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-lifeact-mNeongreen-ORF-3

Plasmid

#241206

Purpose

Middle entry vector for mini-Golden subcloning platform; lifeact-mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

lifeact-mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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SA-linker-P2A-mem-mScarlet-ORF-3

Plasmid

#241188

Purpose

Middle entry vector for mini-Golden subcloning platform; mScarlet

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mScarlet

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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nzCas9n-GSG-linker-P2A-mNeongreen

Plasmid

#241189

Purpose

Middle entry vector for mini-Golden subcloning platform; mNeongreen

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mNeongreen

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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