We narrowed to 5,566 results for: donor

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pDonor_e-attP-H1-g3-Ef1a-Puro-P2A-mCherry

Plasmid

#247160

Purpose

Engineered e-attP donor plasmid expressing puromycin resistance and mCherry with donor binding sgRNA H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Puromycin resistance, mCherry

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor

Plasmid

#92142

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-pCAG-donor(R2Tg)-GFP(intron)

Plasmid

#223248

Purpose

Mammalian expression of the R2Tg RNA donor

Depositor

Wei Li

Article

Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

Insert

R2Tg donor

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS DRR mCherry Donor EF1a BFP

Plasmid

#98896

Purpose

Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-mCherry-GFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF0

Plasmid

#200383

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor (all-in-one) ORF2

Plasmid

#200388

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF1

Plasmid

#200384

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ9663 - ACTB GFP-P2A CLIP Donor

Plasmid

#202688

Purpose

CLIP donor template for integrating GFP into ACTB locus. Encodes GFP with ACTB homology arms.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

CopGFP

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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V309 - pDNR MCS SA lacZ alpha - Donor

Plasmid

#11692

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pHiUGE smFP-V5 donor (all-in-one) ORF0

Plasmid

#200386

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE smFP-V5 donor (all-in-one) ORF1

Plasmid

#200387

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-GZV-SynZiFTR_Down-Tandem donor

Plasmid

#229787

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; ZF10-NS3-p65

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ8079 - IL2RG GFP-P2A CLIP Donor

Plasmid

#202690

Purpose

CLIP donor template for integrating GFP into IL2RG locus.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

CopGFP

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB)

Plasmid

#85798

Purpose

donorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system

Depositor

Roland Friedel

Article

Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-396

Insert

PPP1R12C (PPP1R12C Human)

Use

Human targeting

Mutation

homology arms for knock-in into first intron

Available Since

March 4, 2019

Availability

Academic Institutions and Nonprofits only

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WT1-2A-eGFP PGK PuroR Donor Plasmid

Plasmid

#82333

Purpose

WT1 targeting donor

Depositor

Sean Palecek

Article

Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

Inserts

WT1 5' Arm (WT1 Human)
WT1 3' Arm (WT1 )

Use

CRISPR and TALEN

Tags

2A-eGFP

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

Plasmid

#86843

Purpose

HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

Depositor

Thomas Cech

Article

Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

Insert

FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)

Tags

FLAG-HaloTag

Expression

Mammalian

Promoter

Endogenous TERT promoter

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHiUGE TurboID-HA donor (all-in-one) ORF2

Plasmid

#200385

Purpose

Tagging endogenously expressed proteins with TurboID-HA, C-terminal

Depositor

Scott Soderling

Article

Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

Insert

TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHnS 2comp Donor;smFP-V5 KO;Template ORF0

Plasmid

#240304

Purpose

Donor:smFP-V5 KO:Template

Depositor

Yuki Ogawa

Insert

Template gRNA

Use

AAV

Mutation

NA

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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H516 SONIC KrasG12D-IRES-luciferase donor

Plasmid

#138178

Purpose

CRISPR SONIC: Kras G12D luciferase donor plasmid.

Depositor

Wen Xue

Article

Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

Insert

KrasG12D (Kras Mouse)

Use

Nhej donor

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGS101_EGFP(A206K)-CENP-T(1-242) AAVS donor

Plasmid

#211867

Purpose

CENP-T(1-242) transgene integration at AAVS1 safe harbor locus

Depositor

Iain Cheeseman

Insert

CENP-T(1-242) (CENPT Human, Synthetic)

Use

CRISPR

Mutation

N/A

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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