We narrowed to 6,084 results for: nls

1. pMM0656

   Plasmid

   #129017

   Purpose

   pTEF1-Zif268DBD-CRY2 cassette plasmid flanked by ConL1 and ConR2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   ConL1-pTEF1-SV40NLS-ZiF268DBD-CRY2-tSSA1-ConR2-ColE1-AmpR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMM0317

   Plasmid

   #128979

   Purpose

   Encodes Zif268DBD-CRY2 under pscADH1

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   pscADH-SV40NLS-Zif268DBD-CRY2 (L3)-tscADH1 scTRP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMM0320

   Plasmid

   #128980

   Purpose

   Encodes Zif268DBD-CRY2PHR under pscADH1

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   pscADH1-SV40NLS-ZIF268DBD-CRY2PHR (L3)-tscADH1 TRP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMM0629

   Plasmid

   #129004

   Purpose

   pRNR2-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   ConL1-pRNR2-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMM0521

   Plasmid

   #128984

   Purpose

   Expression plasmid for ZIF268DBD-L3-CRY2

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   SV40NLS-Zif268DBD-CRY2 (L3) scTRP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL-mU6-GFPGO-IRES-mScarletI

   Plasmid

   #136895

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO2.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO-IRES-mScarletI
   

   Use

   Lentiviral

   Tags

   3xNLS on mScarlet

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. nLbCas12a

   Plasmid

   #213745

   Purpose

   For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   nLbCas12a
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, R1138A

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-Jmjd3-F

   Plasmid

   #17440

   Purpose

   Expression of Jmjd3 in mammalian cells

   Depositor

   Kai Ge

   Article

   Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

   Insert

   JMJD3 (Kdm6b Mouse)
   

   Use

   Tags

   6xMyc and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. MTK1_022

   Plasmid

   #123684

   Purpose

   Encodes ConLS and the human A4 insulator as a Type 1 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   Connector LS-A4 insulator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. HERV-Kcon-RT for Split-PE (pJUL3120)

    Plasmid

    #190107

    Purpose

    Expresses HERV-Kcon RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    HERV-Kcon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-CAG-Brainbow3.2-PGK-PuroTK

    Plasmid

    #159928

    Purpose

    Brainbow transgenesis

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

    Inserts

    NFPnls
    mOrange2
    EGFP
    mKate2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL3713 (pTnsC_Pse)

    Plasmid

    #200900

    Purpose

    Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseTnsC
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Marathon-RT pentamutant for Split-PE (pEH107)

    Plasmid

    #190110

    Purpose

    Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    D14R, N26R, D74R, N116K, N197R

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUbi-MAD7

    Plasmid

    #170133

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al J Genet Genomics. 2021 May 3. pii: S1673

    Insert

    MAD7(ErCas12a)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    maize Ubiquitin-1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. LbCas12a-D156R

    Plasmid

    #213744

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCB301TOR-CRISPR

    Plasmid

    #164797

    Purpose

    For gene editing in oomycetes

    Depositor

    Miaoying Tian

    Article

    Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

    Inserts

    PsNLS-hSpCas9
    sgRNA expression cassette
    

    Use

    Oomycete expression

    Tags

    Expression

    Mutation

    Promoter

    HAM34 and RPL41

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCL.139

    Plasmid

    #184994

    Purpose

    Express Cas9-P2A-Eco1RT in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9-P2A-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Human codon optimized RT

    Promoter

    TetOn-3g

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Marathon-RT tetramutant for Split-PE (pEH101)

    Plasmid

    #190109

    Purpose

    Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    D14R, D74R, N116K, N197R

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBTK527

    Plasmid

    #110605

    Purpose

    BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. sABE v3.22 Lenti_2

    Plasmid

    #209556

    Purpose

    pU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRB

    Depositor

    Xue Gao

    Article

    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

    Insert

    SpCas9 sgRNA, TadA-8e (2-76)-2xFRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pU6, pCMV

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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